Natural Products Research Center , Korea Institute of Science and Technology , Gangneung 25451 , Gangwon-do , Republic of Korea.
School of Pharmacy , Sungkyunkwan University , Suwon 16419 , Republic of Korea.
J Nat Prod. 2020 Feb 28;83(2):223-230. doi: 10.1021/acs.jnatprod.9b00543. Epub 2020 Feb 7.
has traditionally been used as an antipyretic, analgesic, and anti-inflammatory agent and as a treatment for uterine and anal prolapse. This study has investigated the potential beneficial effects of this medicinal plant and its components on Alzheimer's disease (AD) with a focus on amyloid beta (Aβ) production and scopolamine-induced memory impairment in mice. An ethanol extract from roots decreased Aβ production in APP-CHO cells [Chinese hamster ovarian (CHO) cells stably expressing amyloid precursor protein (APP)], as determined by an enzyme-linked immunosorbent assay and Western blot analysis. Then, the compounds isolated from were tested for their antiamyloidogenic activities. Four compounds (-) efficiently interrupted Aβ generation by suppressing the level of β-secretase in APP-CHO cells. Moreover, the in vivo experimental results demonstrated that compound improved the cognitive performances of mice with scopolamine-induced disruption on behavioral tests and the expression of memory-related proteins. Taken together, these results suggest that and its constituents are potential agents for preventing or alleviating the symptoms of AD.
传统上被用作解热、镇痛和抗炎剂，以及治疗子宫和肛门脱垂。本研究调查了这种药用植物及其成分对阿尔茨海默病 (AD) 的潜在有益作用，重点是淀粉样蛋白β (Aβ) 的产生和东莨菪碱诱导的小鼠记忆障碍。从 根中提取的乙醇提取物通过酶联免疫吸附试验和 Western blot 分析确定可降低 APP-CHO 细胞（稳定表达淀粉样前体蛋白 (APP) 的中国仓鼠卵巢 (CHO) 细胞）中的 Aβ 产生。然后，测试了从 中分离出的化合物的抗淀粉样蛋白形成活性。四种化合物 (-) 通过抑制 APP-CHO 细胞中 β-分泌酶的水平有效地中断了 Aβ 的产生。此外，体内实验结果表明，化合物 改善了东莨菪碱诱导的行为测试和记忆相关蛋白表达破坏的小鼠的认知表现。总之，这些结果表明 和其成分是预防或缓解 AD 症状的潜在药物。
