Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12/14, 61-704 Poznań, Poland.
Int J Mol Sci. 2020 Feb 4;21(3):1027. doi: 10.3390/ijms21031027.
The application of a new generation of sequencing techniques has revealed that most of the genome has already been transcribed. However, only a small part of the genome codes proteins. The rest of the genome "dark matter" belongs to divergent groups of non-coding RNA (ncRNA), that is not translated into proteins. There are two groups of ncRNAs, which include small and long non-coding RNAs (sncRNA and lncRNA respectively). Over the last decade, there has been an increased interest in lncRNAs and their interaction with cellular components. In this review, we presented the newest information about the human lncRNA interactome. The term lncRNA interactome refers to cellular biomolecules, such as nucleic acids, proteins, and peptides that interact with lncRNA. The lncRNA interactome was characterized in the last decade, however, understanding what role the biomolecules associated with lncRNA play and the nature of these interactions will allow us to better understand lncRNA's biological functions in the cell. We also describe a set of methods currently used for the detection of lncRNA interactome components and the analysis of their interactions. We think that such a holistic and integrated analysis of the lncRNA interactome will help to better understand its potential role in the development of organisms and cancers.
新一代测序技术的应用表明，大部分基因组已经被转录。然而，基因组中只有一小部分编码蛋白质。基因组的其余“暗物质”属于非编码 RNA（ncRNA）的不同群组，即不会被翻译成蛋白质。ncRNA 有两类，包括小 ncRNA 和长 ncRNA（分别为 sncRNA 和 lncRNA）。在过去十年中，人们对 lncRNA 及其与细胞成分的相互作用越来越感兴趣。在这篇综述中，我们介绍了关于人类 lncRNA 相互作用组的最新信息。lncRNA 相互作用组这一术语是指与 lncRNA 相互作用的细胞生物分子，如核酸、蛋白质和肽。lncRNA 相互作用组在过去十年中得到了描述，然而，了解与 lncRNA 相关的生物分子所起的作用以及这些相互作用的性质，将使我们能够更好地理解 lncRNA 在细胞中的生物学功能。我们还描述了目前用于检测 lncRNA 相互作用组成分及其相互作用分析的一组方法。我们认为，对 lncRNA 相互作用组进行这种整体和综合的分析，将有助于更好地理解其在生物发育和癌症中的潜在作用。
