光遗传学在神经移植操作和功能分析中的应用。

Optogenetics for neural transplant manipulation and functional analysis.

机构信息

Technische Universität Dresden, CRTD - Center for Regenerative Therapies at TU Dresden, D-01307, Dresden, Germany.

Technische Universität Dresden, CRTD - Center for Regenerative Therapies at TU Dresden, D-01307, Dresden, Germany; Universitäts-Augenklinik Bonn, University of Bonn, Dep. of Ophthalmology, D-53127, Bonn, Germany.

出版信息

Biochem Biophys Res Commun. 2020 Jun 25;527(2):343-349. doi: 10.1016/j.bbrc.2020.01.141. Epub 2020 Feb 6.

DOI:10.1016/j.bbrc.2020.01.141

PMID:32033753

Abstract

Transplantation of neural stem cells (NSCs) or NSC-derived neurons into the brain is a promising therapeutic approach to restore neuronal function. Rapid progress in the NSCs research field, particularly due to the exploitation of induced pluripotent stem cells (iPSCs), offers great potential and an unlimited source of stem cell-derived neural grafts. Studying the functional integration of these grafts into host brain tissues and their effects on each other have been boosted by the implementation of optogenetic technologies. Optogenetics provides high spatiotemporal functional manipulations of grafted or host neurons in parallel. This review aims to highlight the impact of optogenetics in neural stem cell transplantations.

摘要

将神经干细胞 (NSCs) 或 NSC 衍生的神经元移植到大脑中是恢复神经元功能的一种很有前途的治疗方法。NSCs 研究领域的快速发展，特别是由于诱导多能干细胞 (iPSCs) 的开发，提供了巨大的潜力和无限的干细胞衍生神经移植物来源。通过实施光遗传学技术，促进了这些移植物与宿主脑组织的功能整合及其相互作用的研究。光遗传学提供了对移植或宿主神经元进行高时空功能操作的方法。本综述旨在强调光遗传学在神经干细胞移植中的作用。