College of Forestry, Northwest A&F University, No. 3 Taicheng Road, Yangling 712100, Shaanxi, China.
College of Forestry, Northwest A&F University, No. 3 Taicheng Road, Yangling 712100, Shaanxi, China; Jiangsu Co-Innovation Center for Efficient Processing and Utilization of Forest Products, College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, Jiangsu, China; Key Laboratory of Forestry Genetics & Biotechnology (Nanjing Forestry University), Ministry of Education, Nanjing 210037, China.
Bioresour Technol. 2020 Apr;302:122901. doi: 10.1016/j.biortech.2020.122901. Epub 2020 Jan 28.
The impact of lignin extracted from γ-valerolactone/water (GVL/HO) pretreatment of corn stover on the enzymatic hydrolysis of cellulose was investigated. Two lignin samples were separated and named as GL25 and GL75 according to the amounts of sulfuric acid (25 mM and 75 mM) used in the GVL/HO pretreatment. With the addition of 2 g/L of GL25 and GL75, the glucan conversion of enzymatic hydrolysis of Avicel improved markedly from 28.0% to 37.4% and 31.3%, respectively. Moreover, the improvement of glucan conversion increased when increasing the loadings of GL25 and GL75. A similar observation was made when GVL/HO pretreated corn stover was the substrate. The results of the cellulase adsorption experiments showed that the GLs had a lower maximum cellulase adsorption capacity and binding strength compared to that of acid-insoluble lignin. Further structural characterization of the GLs revealed that they had a low zeta-potential and hydrophobicity, but a high Syringyl/Guaiacyl ratio.
研究了从γ-戊内酯/水（GVL/HO）预处理玉米秸秆中提取的木质素对纤维素酶水解的影响。根据 GVL/HO 预处理中使用的硫酸量（25 mM 和 75 mM），将两种木质素样品分离并分别命名为 GL25 和 GL75。添加 2 g/L 的 GL25 和 GL75 后，微晶纤维素酶水解的葡萄糖转化率分别显著提高到 37.4%和 31.3%。此外，随着 GL25 和 GL75 用量的增加，葡萄糖转化率的提高也随之增加。当以 GVL/HO 预处理的玉米秸秆为底物时，也观察到了类似的结果。纤维素酶吸附实验结果表明，与酸不溶木质素相比，GL 具有较低的最大纤维素酶吸附容量和结合强度。对 GL 的进一步结构表征表明，它们具有较低的动电位和疏水性，但较高的愈创木基/紫丁香基比。
