College of Forestry, Northwest A&F University, No. 3 Taicheng Road, Yangling 712100, Shaanxi, China.
College of Forestry, Northwest A&F University, No. 3 Taicheng Road, Yangling 712100, Shaanxi, China; Co-Innovation Center for Efficient Processing and Utilization of Forest Products, College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, Jiangsu, China.
Bioresour Technol. 2019 Apr;278:440-443. doi: 10.1016/j.biortech.2019.01.141. Epub 2019 Jan 30.
The impact of γ-valerolactone/water (GVL/HO) pretreatment coupled with alkaline post-incubation on the digestibility of cellulose substrates was investigated. When Avicel was pretreated by GVL/HO with HSO as catalyst, the glucan conversion decreased from 27% (untreated Avicel) to 16% due to the esterification of cellulose after pretreatment. After incubation of GVL/HO pretreated Avicel with 1% NaOH at 50 °C for 1 h, the glucan conversion enhanced to 20%. When corn stover (CS) pretreated by GVL/HO with 25 mM HSO was used as substrate, alkaline post-incubation removed 72% and 50% of the hemicellulose and lignin, respectively, and increased the glucan conversion by 1.9-fold consequently. Moreover, higher cellulases adsorption capacity, affinity and binding strength on GVL/HO pretreated CS was achieved after alkaline post-incubation. These results indicated that the improved digestibility of GVL/HO pretreated lignocellulose by alkaline post-incubation was attributed to the depletion of ester groups on cellulose and removal of hemicellulose and lignin.
研究了γ-戊内酯/水（GVL/HO）预处理与碱性后孵育相结合对纤维素底物消化率的影响。当使用 HSO 作为催化剂用 GVL/HO 预处理微晶纤维素时，由于预处理后纤维素的酯化，葡萄糖转化率从 27%（未处理的微晶纤维素）降低到 16%。用 1% NaOH 在 50°C 下孵育 GVL/HO 预处理的微晶纤维素 1 小时后，葡萄糖转化率提高到 20%。当用 25mM HSO 预处理的 GVL/HO 处理玉米秸秆（CS）作为底物时，碱性后孵育分别去除了 72%和 50%的半纤维素和木质素，从而使葡萄糖转化率提高了 1.9 倍。此外，碱性后孵育后，更高的纤维素酶在 GVL/HO 预处理 CS 上的吸附量、亲和力和结合强度。这些结果表明，碱性后孵育通过耗尽纤维素上的酯基和去除半纤维素和木质素来提高 GVL/HO 预处理木质纤维素的消化率。
