Pacheco Fabiana Santos, Prata Rhana Berto da Silva, Brandão Sheila Santos, Ferreira Helen, Rodrigues Thaís Fernanda, Brandão Dos Santos Jéssica, da Silva Camila Oliveira, Tavares Isabella Forasteiro, Mendes Mayara Abud, Rodrigues Ana Carolina Duarte Pereira, Machado Alice de Miranda, Nery José Augusto da Costa, Amadeu Thaís Porto, Moraes Milton Ozório, Sarno Euzenir Nunes, Schmitz Veronica
Leprosy Laboratory, Oswaldo Cruz Institute, FIOCRUZ, Rio de Janeiro, Rio de Janeiro 21040-900, Brazil.
Cellular Microbiology Laboratory, Oswaldo Cruz Institute, FIOCRUZ, Rio de Janeiro, Rio de Janeiro 21040-900, Brazil; and.
Immunohorizons. 2020 Feb 7;4(2):47-56. doi: 10.4049/immunohorizons.1900088.
Erythema nodosum leprosum (ENL) is an inflammatory complication in leprosy. Yet, the involvement of ENL neutrophils in the inflammatory response against remains poorly explored. Our primary aim was to investigate the utility of the surface expression of neutrophil IL-10R1 as an ENL biomarker and, secondarily, to evaluate whether leprosy or healthy -stimulated neutrophils produce cytokines and are able to respond to IL-10. We, in this study, describe a subpopulation of circulating neutrophils of ENL patients that exclusively expressed IL-10R1, providing evidence that IL-10R1 neutrophils are present in ENL lesions. It was also found that ENL neutrophils, but not those of nonreactional leprosy controls, were able to secret detectable levels of TNF ex vivo and the addition of IL-10 blocked TNF release. It was likewise observed that stimulated, healthy neutrophils expressed IL-10R1 in vitro, and ENL-linked cytokines were released by -cultured neutrophils in vitro. Moreover, consistent with the presence of a fully functional IL-10R, the addition of IL-10 prevented the release of -induced cytokines. Most importantly, dead revealed its superior capacity to induce and in primary neutrophils over live , suggesting that may hamper the inflammatory machinery as an immune escape mechanism.
结节性红斑麻风（ENL）是麻风病中的一种炎症并发症。然而，ENL中性粒细胞在针对……的炎症反应中的作用仍未得到充分研究。我们的主要目的是研究中性粒细胞IL-10R1的表面表达作为ENL生物标志物的效用，其次是评估麻风病患者或健康人刺激的中性粒细胞是否产生细胞因子以及是否能够对IL-10作出反应。在本研究中，我们描述了ENL患者循环中性粒细胞的一个亚群，该亚群专门表达IL-10R1，这证明IL-10R1阳性中性粒细胞存在于ENL病变中。还发现，ENL中性粒细胞而非无反应性麻风病对照的中性粒细胞能够在体外分泌可检测水平的TNF，并且添加IL-10可阻断TNF释放。同样观察到，受刺激的健康中性粒细胞在体外表达IL-10R1，并且与ENL相关的细胞因子在体外由培养的中性粒细胞释放。此外，与存在功能完全正常的IL-10R一致，添加IL-10可阻止诱导的细胞因子释放。最重要的是，死亡的……显示出其在诱导原代中性粒细胞产生……和……方面比活的……具有更强的能力，这表明……可能作为一种免疫逃逸机制阻碍炎症机制。
