Department of Food Chemistry, National Institute of Chemistry, Hajdrihova 19, 1001, Ljubljana, Slovenia.
Faculty of Chemistry and Chemical Technology, University of Ljubljana, Večna pot 113, 1000, Ljubljana, Slovenia.
Sci Rep. 2020 Feb 7;10(1):2163. doi: 10.1038/s41598-020-59093-1.
During sample preparation and analysis, samples are coming in contact with different labware materials. By four unrelated analytical (phytochemical and pharmaceutical) case-studies and employing different analytical techniques, we demonstrated the potential misinterpretation of analytical results due to the use of contaminants-leaching labware during sample handling. Oleamide, a common polymer lubricant and a bioactive compound, was identified as a main analytical interference, leaching from different labware items into solvents, recognised as chemically compatible with the tested polymer material. Moreover, anti-inflammatory effect of oleamide at 100 μg mL and considerable pro-inflammatory effect of the plastic syringe extractables (containing oleamide) at the same level were shown in a TLR4-based bioassay. Taking these results into account, together with the fact that oleamide can be a compound of natural origin, we would like to notify the professional public regarding the possible erroneous oleamide-related analytical and bioassay results due to the use of oleamide-leaching labware. Researchers are alerted to double check the real source of oleamide (labware or natural extract), which will prevent further reporting of false results. Analysis of procedural blanks with de-novo developed UHPLC-ESI-MS method is, among some other strategies, proposed for detection of oleamide interference and avoidance of misleading results of certain analyses.
在样品制备和分析过程中，样品会与不同的实验室器具材料接触。通过四个不相关的分析（植物化学和药物）案例研究，并采用不同的分析技术，我们证明了由于在样品处理过程中使用了含有污染物的浸出实验室器具，可能会导致分析结果出现误解。油酰胺是一种常见的聚合物润滑剂和生物活性化合物，被鉴定为主要的分析干扰物，从不同的实验室器具材料浸出到溶剂中，被认为与测试的聚合物材料在化学上是相容的。此外，在基于 TLR4 的生物测定中，在 100μg/mL 下，油酰胺显示出抗炎作用，而在相同水平下，塑料注射器浸出物（含有油酰胺）显示出相当大的促炎作用。考虑到这些结果，以及油酰胺可能是天然来源的化合物这一事实，我们希望通知专业公众注意由于使用浸出油酰胺的实验室器具而可能导致的错误的油酰胺相关分析和生物测定结果。研究人员被提醒要仔细检查油酰胺的真正来源（实验室器具或天然提取物），这将防止进一步报告错误的结果。使用新开发的 UHPLC-ESI-MS 方法分析程序空白是检测油酰胺干扰和避免某些分析产生误导性结果的一些策略之一。
