Braun Marcus, Esposito Tiago, Huettel Bruno, Harand Andrea Pedrosa
Laboratory of Plant Cytogenetics and Evolution, Department of Botany, Center of Biosciences, Federal University of Pernambuco, R. Prof. Moraes Rego, s/n, CDU, Recife, PE, 50670-420, Brazil.
Max Planck Genome Centre Cologne, Max Planck Institute for Plant Breeding Research, Carl-von-Linne-Weg 10, 50829, Cologne, Germany.
Mol Biol Rep. 2019 Apr;46(2):2529-2532. doi: 10.1007/s11033-019-04622-z. Epub 2019 Jan 28.
Microsatellites markers were developed for Paypayrola blanchetiana (Violaceae), a near-dispersing forest tree forming aggregated populations, to investigate genetic diversity and gene flow among subpopulations in a fragmented environment. Next generation sequencing (Illumina platform) was used to develop ten nuclear microsatellite loci and one plastid microsatellite locus that amplify in P. blanchetiana. Polymorphism was tested in two subpopulations separated by a distance of approximately 11 km. The identified loci contained between two and five alleles per locus. Observed heterozygosity ranged between 0.063 and 0.563 in both subpopulations, while expected heterozygosity ranged from 0.063 to 0.567 in the first, and 0.063-0.627 in the second subpopulation. The microsatellites are among the first in the family Violaceae and will be useful for population genetic studies in this species. Amplification was successful in one further Paypayrola species from Amazonia, which suggest a wider usefulness of the present markers.
为白花赛金莲木（堇菜科）开发了微卫星标记，白花赛金莲木是一种近乎随机分布的森林树木，形成聚集种群，旨在研究碎片化环境中各亚种群间的遗传多样性和基因流。利用二代测序（Illumina平台）开发了10个能在白花赛金莲木中扩增的核微卫星位点和1个质体微卫星位点。在相隔约11公里的两个亚种群中测试了多态性。所鉴定的位点每个位点包含2至5个等位基因。在两个亚种群中，观察到的杂合度在0.063至0.563之间，而在第一个亚种群中，预期杂合度在0.063至0.567之间，在第二个亚种群中为0.063 - 0.627。这些微卫星标记是堇菜科中的首批标记之一，将有助于该物种的群体遗传学研究。在来自亚马逊地区的另一种赛金莲木中扩增成功，这表明当前标记具有更广泛的用途。
