Institute of Biotechnology, Federal University of Uberlandia, Av. Amazonas, Bloco 2E, Campus Umuarama, 38400-902 Uberlandia, MG, Brazil.
Institute of Biotechnology, Federal University of Uberlandia, Av. Amazonas, Bloco 2E, Campus Umuarama, 38400-902 Uberlandia, MG, Brazil.
Int J Biol Macromol. 2020 Apr 15;149:1241-1251. doi: 10.1016/j.ijbiomac.2020.02.028. Epub 2020 Feb 5.
The main systemic alterations present in bothropic envenomation are hemostasis disorders, for which the conventional treatment is based on animal-produced antiophidic sera. We have developed a neutralizing antibody against Bothrops pauloensis (B. pauloensis) venom, which is member of the genus most predominant in snakebite accidents in Brazil. Subsequently, we expressed this antibody in plants to evaluate its enzymatic and biological activities. The ability of single-chain variable fragment (scFv) molecules to inhibit fibrinogenolytic, azocaseinolytic, coagulant and hemorrhagic actions of snake venom metalloproteinases (SVMPs) contained in B. pauloensis venom was verified through proteolytic assays. The antibody neutralized the toxic effects of envenomation, particularly those related to systemic processes, by interacting with one of the predominant classes of metalloproteinases. This novel molecule is a potential tool with great antivenom potential and provides a biotechnological antidote to snake venom due to its broad neutralizing activity.
两种矛头蝮蛇属蛇毒引起的全身性改变主要为止血功能紊乱，针对这一症状的常规治疗方法是基于动物源性抗蛇毒血清。我们已经开发出一种针对巴西蛇伤事故中最常见的矛头蝮蛇属蛇毒（B. pauloensis 蛇毒）的中和抗体。随后，我们在植物中表达了这种抗体，以评估其酶和生物学活性。通过蛋白水解分析，验证了单链可变片段（scFv）分子抑制 B. pauloensis 蛇毒金属蛋白酶（SVMPs）纤维蛋白原水解、偶氮酪蛋白水解、凝血和出血作用的能力。该抗体通过与主要的金属蛋白酶之一相互作用，中和了蛇毒的毒性作用，特别是与全身过程相关的毒性作用。这种新型分子具有广阔的中和活性，是一种有很大抗蛇毒潜力的潜在工具，为蛇毒提供了一种生物技术解毒剂。
