Laboratory of Fish Nutrition, School of Marine Sciences, Ningbo University, Ningbo, 315211, PR China.
Ningbo Economic Technical Development Area Bolun Marine Surveyors Office, Ningbo, 315800, PR China.
Fish Shellfish Immunol. 2020 Apr;99:184-189. doi: 10.1016/j.fsi.2020.02.009. Epub 2020 Feb 6.
The intestine is the primary target of pathogenic microbes during invasion. However, the interaction of Vibrio parahaemolyticus (V. parahaemolyticus) with intestinal epithelial cells and its effects on the intestinal function of Litopenaeus vannamei (L. vannamei) are poorly studied. Therefore, the aim of this study was to investigate the influence of V. parahaemolyticus infection on intestinal barrier function and nutrient absorption in L. vannamei. In the present study, a total of 90 shrimp were randomly divided into two groups including the control group and V. parahaemolyticus infection group (final concentration of 1 × 10 CFU/mL), with three replicates per group. The result showed that compared with the control group, V. parahaemolyticus infection increased (P < 0.05) serum diamine oxidase activity and endotoxin quantification, and down-regulated (P < 0.05) the mRNA levels of intestinal peroxinectin, integrin, midline fasciclin at 48 h and 72 h; V. parahaemolyticus infection decreased (P < 0.05) the mRNA expression of intestinal amino acid transporter (CAT1, EAAT3 and ASCT1) and glucose transporter (SGLT-1, GLUT) at 24 h, 48 h and 72 h, and increased (P < 0.05) serum glucose and amino acid (Asp, Thr, Ser, Glu, Gly, Ala, Val, Ile, Leu, Tyr, Phe, Lys, His and Arg) concentration at 24 h. The results indicated that V. parahaemolyticus infection increased intestinal permeability, inhibited absorption of glucose and amino acid in L. vannamei.
肠道是病原菌入侵时的主要靶标。然而，副溶血弧菌（Vibrio parahaemolyticus，V. parahaemolyticus）与肠上皮细胞的相互作用及其对凡纳滨对虾（Litopenaeus vannamei，L. vannamei）肠道功能的影响尚未得到充分研究。因此，本研究旨在探讨副溶血弧菌感染对凡纳滨对虾肠道屏障功能和营养吸收的影响。本研究中，将 90 只虾随机分为对照组和副溶血弧菌感染组（终浓度为 1×10 CFU/mL），每组设 3 个重复。结果显示，与对照组相比，副溶血弧菌感染增加了（P<0.05）血清二胺氧化酶活性和内毒素含量，并下调了（P<0.05）48 h 和 72 h 时肠穿孔素、整合素、中线粘连蛋白的 mRNA 水平；副溶血弧菌感染降低了（P<0.05）24 h、48 h 和 72 h 时肠氨基酸转运体（CAT1、EAAT3 和 ASCT1）和葡萄糖转运体（SGLT-1、GLUT）的 mRNA 表达，增加了（P<0.05）24 h 时血清葡萄糖和氨基酸（Asp、Thr、Ser、Glu、Gly、Ala、Val、Ile、Leu、Tyr、Phe、Lys、His 和 Arg）浓度。这些结果表明，副溶血弧菌感染增加了肠道通透性，抑制了凡纳滨对虾对葡萄糖和氨基酸的吸收。
