反胰蛋白酶 GP2 和抗酿酒酵母抗体在感染副结核病反刍动物中的作用：对克罗恩病免疫发病机制的更好理解。

Pancreatic anti-GP2 and anti-Saccharomyces cerevisiae antibodies in ruminants with paratuberculosis: A better understanding of the immunopathogenesis of Crohn's disease.

机构信息

Department of Rheumatology and Clinical Immunology, Faculty of Medicine, School of Health Sciences, University of Thessaly, 41110 Larissa, Greece.

出版信息

Clin Res Hepatol Gastroenterol. 2020 Oct;44(5):778-785. doi: 10.1016/j.clinre.2019.12.013. Epub 2020 Feb 5.

DOI:10.1016/j.clinre.2019.12.013

PMID:32035824

Abstract

INTRODUCTION

Ruminants (cattle and sheep) with Mycobacterium avium (MAP)-induced paratuberculosis (ptb), the ruminant model of Crohn's disease (CD), exhibit pancreatic specific autoantibodies (PAB) against GP2 but not against CUZD1. Since anti-Saccharomyces cerevisiae antibodies (ASCAs) is a CD marker, we tested MAP-infected ptb ruminants for ASCA, and compared them with ruminants lacking evidence of anti-MAP serology or with ruminants, which were positive for anti-GP2 antibodies.

MATERIAL AND METHODS

A total of 98 samples from ruminants (48 cattle and 50 sheep) were studied. IgG anti-MAP antibodies, and CD-related ASCA and anti-GP2 antibodies were tested by modified ELISAs.

RESULTS

Nine cattle (18.75%) and 20 sheep (40%) were suffered from ptb. ASCA antibodies were present in 21/48 (43.7%) cattle and 10/50 (20%) sheep while anti-GP2 antibodies were present in 14/48 (29.2%) cattle, and 8/50 (16%) sheep. ASCA antibodies were more prevalent in anti-MAP antibody positive (14/29, 48.3%) than in anti-MAP negative ruminants (17/69, 24.6%, P=0.022) and also in anti-GP2 antibody positive (13/23, 56.5%) than in anti-GP2 negative ruminants (18/75, 24%, P=0.003). No association between ASCA and anti-MAP antibody concentrations were found (r=0.159, P=0.117). A significant association between ASCA and anti-GP2 antibody concentration were observed (r=0.211 and P=0.037).

CONCLUSION

ASCA are present in a significant proportion of ruminants with ptb and correlate with anti-GP2 antibody positivity, a finding further supporting the notion that Crohn's disease and ptb share common immunological mechanisms of antigen-driven loss of self-tolerance.

摘要

简介

患有由分枝杆菌（牛和羊）引起的副结核病（ptb）的反刍动物（牛和羊），是克罗恩病（CD）的反刍动物模型，针对 GP2 表现出胰腺特异性自身抗体（PAB），但不针对 CUZD1。由于抗酿酒酵母抗体（ASCA）是 CD 的标志物，我们测试了感染 MAP 的 ptb 反刍动物的 ASCA，并将其与缺乏抗 MAP 血清学证据的反刍动物或抗 GP2 抗体阳性的反刍动物进行了比较。

材料和方法

共研究了 98 份来自反刍动物（48 头牛和 50 只羊）的样本。通过改良 ELISA 检测 IgG 抗 MAP 抗体、CD 相关 ASCA 和抗 GP2 抗体。

结果

9 头牛（18.75%）和 20 只羊（40%）患有副结核病。21/48（43.7%）头牛和 10/50（20%）只羊存在 ASCA 抗体，14/48（29.2%）头牛和 8/50（16%）只羊存在抗 GP2 抗体。抗 MAP 抗体阳性（14/29，48.3%）的反刍动物比抗 MAP 抗体阴性（17/69，24.6%）的反刍动物中更普遍存在 ASCA 抗体（P=0.022），抗 GP2 抗体阳性（13/23，56.5%）的反刍动物比抗 GP2 抗体阴性（18/75，24%）的反刍动物中更普遍存在 ASCA 抗体（P=0.003）。ASCA 与抗 MAP 抗体浓度之间无相关性（r=0.159，P=0.117）。ASCA 与抗 GP2 抗体浓度之间存在显著相关性（r=0.211 和 P=0.037）。