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健康猪的肝病毒组揭示了多样化的小型 ssDNA 病毒基因组。

Liver virome of healthy pigs reveals diverse small ssDNA viral genomes.

机构信息

Laboratório de Virologia Veterinária, Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul, Brazil.

Departamento de Biotecnologia, Universidade Federal da Paraíba (UFPB), João Pessoa, Paraíba, Brazil.

出版信息

Infect Genet Evol. 2020 Jul;81:104203. doi: 10.1016/j.meegid.2020.104203. Epub 2020 Feb 6.

DOI:10.1016/j.meegid.2020.104203
PMID:32035977
Abstract

Brazil is a major exporter of pork meat worldwide. Swine liver is a common ingredient in food consumed by humans, thus emphasizing the importance of evaluating the presence of associated pathogens in swine liver. To obtain knowledge, this study aimed to provide insights into the viral communities of livers collected from slaughtered pigs from southern Brazil. The 46 livers were processed and submitted for high-throughput sequencing (HTS). The sequences were most closely related to Anelloviridae, Circoviridae and Parvoviridae families. The present work also describes the first Brazilian PCV1 and the first PPV6 and PPV7 from South America. Virus frequencies revelead 63% of samples positive for TTSuV1, 71% for TTSuVk2, 10.8% for PCV, 13% for PPV and 6% for PBov. This report addresses the diversity of the liver virome of healthy pigs and expands the number of viruses detected, further characterizing their genomes to assist future studies.

摘要

巴西是全球猪肉的主要出口国。猪肝是人类食用的常见食物成分,因此强调评估猪肝脏中相关病原体的存在非常重要。为了获得知识,本研究旨在深入了解来自巴西南部屠宰猪的肝脏中的病毒群落。对 46 个肝脏进行了处理并进行了高通量测序(HTS)。这些序列与圆环病毒科、细小病毒科和细小病毒科的关系最为密切。本工作还描述了来自南美的第一株 PCV1、第一株 PPV6 和第一株 PPV7。病毒频率显示,63%的样品对 TTSuV1 呈阳性,71%对 TTSuVk2 呈阳性,10.8%对 PCV 呈阳性,13%对 PPV 呈阳性,6%对 PBov 呈阳性。本报告阐述了健康猪肝脏病毒组的多样性,并增加了检测到的病毒数量,进一步对其基因组进行了特征描述,以帮助未来的研究。

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