Department of Veterinary Pathobiology, College of Veterinary Medicine, Oklahoma State University (OSU), Stillwater, OK, 74078, USA; Laboratório de Virologia, Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Rio Grande do Sul, Brazil.
Department of Veterinary Pathobiology, College of Veterinary Medicine, Oklahoma State University (OSU), Stillwater, OK, 74078, USA.
Virology. 2021 Apr;556:87-95. doi: 10.1016/j.virol.2021.01.006. Epub 2021 Jan 30.
In the United States, show pigs are raised to compete in agricultural events. These animals are usually raised in small herds with extensive human, domestic, and wild animal contact. Therefore, pathogen monitoring in this animal category is critical for improved disease surveillance and preparedness. This study characterized the virome of healthy show pigs using high-throughput sequencing using pooled serum samples from 2018 or 2019 (200 samples each pool). Results demonstrated the presence of DNA viral families (Parvoviridae, Circoviridae, and Herpesviridae) and RNA families (Arteriviridae, Flaviviridae, and Retroviridae). Twenty-three viral species were identified, including the first detection of porcine bufavirus in the US. Moreover, important swine pathogens identified included porcine reproductive and respiratory syndrome virus, atypical porcine pestivirus, and porcine circovirus (PCV). Additionally, complete coding genomes of 17 viruses from the Parvoviridae, Anelloviridae, and Circoviridae families were retrieved and included the first near full-length genomes of US Ungulate bocaparvovirus 3 species.
在美国，展示猪是为了参加农业活动而饲养的。这些动物通常在小群体中饲养，与人类、家养动物和野生动物有广泛的接触。因此，对这一动物类别的病原体监测对于改善疾病监测和防范至关重要。本研究使用高通量测序技术，对来自 2018 年或 2019 年（每个池 200 个样本）的混合血清样本，对健康展示猪的病毒组进行了特征描述。结果表明存在 DNA 病毒家族（细小病毒科、圆环病毒科和疱疹病毒科）和 RNA 病毒家族（动脉炎病毒科、黄病毒科和逆转录病毒科）。鉴定出 23 种病毒，包括在美国首次检测到的猪 bufavirus。此外，还鉴定出了重要的猪病原体，包括猪繁殖与呼吸综合征病毒、非典型猪瘟病毒和猪圆环病毒（PCV）。此外，还从细小病毒科、圆环病毒科和圆环病毒科中回收了 17 种病毒的完整编码基因组，并包括美国无脊椎动物 bocaparvovirus 3 种的首个近全长基因组。
