College of Animal Science and Technology, Key Laboratory of Animal Origin Food Production and Safety Guarantee of Jiangsu Province, Jiangsu Collaborative Innovation Center of Meat Production and Processing, Quality and Safety Control, Joint International Research Laboratory of Animal Health and Food Safety, National Experimental Teaching Demonstration Center of Animal Science, Nanjing Agricultural University, Nanjing 210095, China.
College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
Food Chem. 2020 Jun 30;316:126271. doi: 10.1016/j.foodchem.2020.126271. Epub 2020 Jan 23.
The current study was designed to investigate the physiochemical properties, protein and metabolite profiles of muscle exudate obtained from chicken breast fillets affected by wooden breast (WB) myopathy. Twenty-four fillets were categorized into varying degrees of WB condition including normal, moderate and severe. Results indicated that exudate loss, free hemoglobin concentration, protein and lipid oxidation were affected by WB myopathy. Electrophoresis analysis showed eight distinct protein bands of differential relative abundance in WB samples compared with the normal, and the identified proteins were mostly involved in carbohydrate metabolic process. H nuclear magnetic resonance-based metabolomics identified eleven metabolites including amino acids, nucleotides and organic acid as the most influential metabolites affected by WB myopathy. Overall, this study shows differential molecular profiles of myopathic chicken muscle exudate, and provides a valuable resource for further recognition of WB myopathy.
本研究旨在探究受木质鸡胸肉（WB）肌病影响的鸡胸肉片的生理化学特性、蛋白质和代谢物图谱。24 片鸡胸肉根据 WB 病变的不同程度分为正常、中度和重度。结果表明，渗出损失、游离血红蛋白浓度、蛋白质和脂质氧化均受 WB 肌病影响。电泳分析显示，与正常组相比，WB 组有 8 条明显差异相对丰度的蛋白质条带，鉴定出的蛋白质大多与碳水化合物代谢过程有关。基于 H 核磁共振的代谢组学鉴定出 11 种代谢物，包括氨基酸、核苷酸和有机酸，这些代谢物是受 WB 肌病影响的最主要的代谢物。总的来说，本研究显示了肌病鸡肉渗出物的差异分子图谱，为进一步认识 WB 肌病提供了有价值的资源。
