Rice C L, Cunningham D A, Taylor A W, Paterson D H
Faculty of Physical Education, University of Western Ontario, Canada.
Eur J Appl Physiol Occup Physiol. 1988;58(1-2):165-70. doi: 10.1007/BF00636621.
Contractile and histochemical properties of the triceps surae were compared in 16 males and 4 females aged 20 to 49 years. Surface electrical stimulation was used to determine twitch, tetanic and fatigue parameters. From these tests, twitch tension (Pt), time to peak tension (TPT), half relaxation time (1/2 RT), tetanic tensions at 10, 20 and 50 Hz and an index of fatigue (FI) were calculated. A maximal voluntary contraction (MVC) was also performed. Muscle samples from the belly of the lateral gastrocnemius were obtained using the needle biopsy technique. The samples were treated histochemically for myosin ATPase and NADH-tetrazolium reductase in order to classify the fibres as either Type I, slow twitch (ST) or Type II, fast twitch (FT) and to determine fibre areas. Correlations were performed between the grouped male and female contractile and histochemical variables. The results demonstrated significant positive relationships demonstrated significant positive relationships between percentage of ST fibres (%ST) and TPT (r = 0.49), and %ST and the ratio of tetanic forces at 10 Hz to 50 Hz (Po10/Po50) (r = 0.55). No significant relationships were obtained for Pt, 1/2 RT, MVC or FI with any histochemical parameter. The results suggest that fibre type distribution determined using myosin ATPase is related to electrically stimulated isometric contractile speeds and not to voluntary force generation (MVC) or electrically induced fatigue.
对16名年龄在20至49岁之间的男性和4名女性的腓肠肌收缩和组织化学特性进行了比较。采用表面电刺激来测定单收缩、强直收缩和疲劳参数。通过这些测试，计算出单收缩张力（Pt）、达到峰值张力的时间（TPT）、半松弛时间（1/2 RT）、10Hz、20Hz和50Hz时的强直张力以及疲劳指数（FI）。还进行了最大自主收缩（MVC）测试。使用针吸活检技术从外侧腓肠肌肌腹获取肌肉样本。对样本进行组织化学处理，检测肌球蛋白ATP酶和NADH-四氮唑还原酶，以便将纤维分类为I型（慢肌纤维，ST）或II型（快肌纤维，FT），并确定纤维面积。对分组后的男性和女性的收缩及组织化学变量进行了相关性分析。结果表明，慢肌纤维百分比（%ST）与TPT（r = 0.49）以及%ST与10Hz和50Hz时强直力的比值（Po10/Po50）（r = 0.55）之间存在显著正相关。Pt、1/2 RT、MVC或FI与任何组织化学参数均未发现显著相关性。结果表明，使用肌球蛋白ATP酶确定的纤维类型分布与电刺激等长收缩速度有关，而与自主力量产生（MVC）或电诱导疲劳无关。
