Abaturov Aleksandr, Nikulina Anna
Department of Pediatrics and Medical Genetics, State Institution "Dnepropetrovsk Medical Academy of the Ministry of Health of Ukraine", Dnipro, Ukraine.
Acta Medica (Hradec Kralove). 2019;62(4):150-155. doi: 10.14712/18059694.2020.4.
To reduce the risk of insulin resistance in obesity in children with lactase gene genotypes, we studied the factors that stimulate the chronic inflammatory process.
109 children 6-18 years of age were investigated. The main group (n = 56) was presented by children with signs of insulin-resistant obesity according to the criteria of the European Society of Endocrinology and the Pediatric Endocrine Society. The control group (n = 53) included obese children without insulin resistance. A comprehensive clinical examination, food diary analysis, genotyping of the lactase gene by means of the polymerase chain reaction, the Immunochemical Test Method with Electrochemiluminescent Detection of basal insulinemia, Hydrogen breath test with lactose load, sequential analysis, ROC analysis were carried out.
Clinical manifestations of lactose maldigestion in a child increased the risk of possible insulin resistance (prognostic coefficient (PC +2.6), as well as the presence of the lactase C/C 13910 gene genotype (PC +5.8) did. The genotype C/T 13910 in children had a protective effect on the risk of obesity (PC -2.9). The lowest risk of insulin-resistant obesity in observed among children with the genotype T/T 13910 (PC -12).
The presence of the C/C 13910 genotype of the lactase gene is the main factor formation of insulin resistance in children's obesity. What is known? The genotype C/C 13910 of the lactase gene as a risk factor for the chronic inflammatory process in the body. What is New? Genotype C/C 13910 of the lactase gene as a risk factor for insulin-resistant obesity in children.
为降低乳糖酶基因基因型儿童肥胖症中胰岛素抵抗的风险，我们研究了刺激慢性炎症过程的因素。
对109名6至18岁的儿童进行了调查。主要组（n = 56）为根据欧洲内分泌学会和儿科内分泌学会标准患有胰岛素抵抗性肥胖症迹象的儿童。对照组（n = 53）包括无胰岛素抵抗的肥胖儿童。进行了全面的临床检查、食物日记分析、通过聚合酶链反应对乳糖酶基因进行基因分型、采用电化学发光检测基础胰岛素血症的免疫化学测试方法、乳糖负荷氢呼气试验、序列分析、ROC分析。
儿童乳糖消化不良的临床表现增加了可能出现胰岛素抵抗的风险（预后系数（PC +2.6）），乳糖酶C/C 13910基因基因型的存在也有此作用（PC +5.8）。儿童中的C/T 13910基因型对肥胖风险有保护作用（PC -2.9）。在基因型为T/T 13910的儿童中观察到胰岛素抵抗性肥胖的风险最低（PC -12）。
乳糖酶基因C/C 13910基因型的存在是儿童肥胖症中胰岛素抵抗形成的主要因素。已知什么？乳糖酶基因的C/C 13910基因型是体内慢性炎症过程的危险因素。新发现是什么？乳糖酶基因的C/C 13910基因型是儿童胰岛素抵抗性肥胖的危险因素。
