Guangdong Provincial Key Laboratory of Animal Nutrition and Regulation, College of Animal Science, South China Agricultural University, Guangzhou 510642, China.
Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China.
Poult Sci. 2020 Feb;99(2):1124-1134. doi: 10.1016/j.psj.2019.10.041. Epub 2019 Dec 12.
Curcumin has antioxidant functions, regulates the intestinal microbial composition, and alleviates mycotoxin toxicity. The present study aimed to explore whether curcumin could alleviate ochratoxin A (OTA)-induced liver injury via the intestinal microbiota. A total of 720 mixed-sex 1-day-old White Pekin ducklings were randomly assigned into 4 groups: CON (control group, without OTA), OTA (fed a diet with 2 mg/kg OTA), CUR (ducks fed a diet with 400 mg/kg curcumin), and OTA + CUR (2 mg/kg OTA plus 400 mg/kg curcumin). Each treatment consisted of 6 replicates and 30 ducklings per replicate. Treatment lasted for 21 D. Results were analyzed by a two-tailed Student t test between 2 groups. Our results demonstrated that OTA treatment had the highest serum low-density lipoprotein (LDL) level among 4 groups. Compared with OTA group, OTA + CUR decreased serum LDL level (P < 0.05). OTA decreased liver catalase (CAT) activity in ducks (P < 0.05), while addition of curcumin in OTA group increased liver CAT activity (P < 0.05). 16S ribosomal RNA sequencing suggested that curcumin increased the richness indices (ACE index) and diversity indices (Simpson index) compared with OTA group (P < 0.05) and recovered the OTA-induced alterations in composition of the intestinal microbiota. Curcumin supplementation relieved the decreased abundance of butyric acid producing bacteria, including blautia, butyricicoccus, and butyricimonas, induced by OTA (P < 0.05). OTA also significantly influenced the metabolism of the intestinal microbiota, such as tryptophan metabolism and glyceropholipid metabolism. Curcumin could alleviate the upregulation of oxidative stress pathways induced by OTA. OTA treatment also increased SREBP-1c expression (P < 0.05). The curcumin group had the lowest expression of FAS and PPARG mRNA (P < 0.05) and the highest expression of NRF2 and HMOX1 mRNA. These results indicated that curcumin could alleviate OTA-induced oxidative injury and lipid metabolism disruption by modulating the cecum microbiota.
姜黄素具有抗氧化功能，可调节肠道微生物组成，减轻霉菌毒素毒性。本研究旨在探讨姜黄素是否可以通过肠道微生物群来减轻黄曲霉毒素 A (OTA) 诱导的肝损伤。将 720 只雌雄混合 1 日龄白羽北京鸭随机分为 4 组：CON（对照组，无 OTA）、OTA（饲料中添加 2mg/kg OTA）、CUR（饲料中添加 400mg/kg 姜黄素）和 OTA+CUR（2mg/kg OTA 加 400mg/kg 姜黄素）。每个处理组包含 6 个重复，每个重复 30 只鸭。处理持续 21 天。通过两组之间的双尾学生 t 检验分析结果。结果表明，4 组中 OTA 处理组的血清低密度脂蛋白（LDL）水平最高。与 OTA 组相比，OTA+CUR 降低了血清 LDL 水平（P < 0.05）。OTA 降低了鸭肝脏过氧化氢酶（CAT）活性（P < 0.05），而在 OTA 组中添加姜黄素则增加了肝脏 CAT 活性（P < 0.05）。16S 核糖体 RNA 测序表明，与 OTA 组相比，姜黄素增加了丰富度指数（ACE 指数）和多样性指数（辛普森指数）（P < 0.05），并恢复了 OTA 诱导的肠道微生物群组成的改变。姜黄素补充剂缓解了由 OTA 引起的丁酸产生菌（包括布劳氏菌、丁酸球菌和丁酸单胞菌）丰度的降低（P < 0.05）。OTA 还显著影响了肠道微生物群的代谢，如色氨酸代谢和甘油磷脂代谢。姜黄素可以减轻 OTA 诱导的氧化应激途径的上调。OTA 处理还增加了 SREBP-1c 的表达（P < 0.05）。姜黄素组 FAS 和 PPARG mRNA 的表达最低（P < 0.05），NRF2 和 HMOX1 mRNA 的表达最高。这些结果表明，姜黄素通过调节盲肠微生物群可以缓解 OTA 诱导的氧化损伤和脂质代谢紊乱。
