Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Key Laboratory of Animal Nutrition and Feed Science (South China) of Ministry of Agriculture, State Key Laboratory of Livestock and Poultry Breeding, Guangdong Pubic Laboratory of Animal Breeding and Nutrition, Guangdong Key Laboratory of Animal Breeding and Nutrition, Guangzhou 510640, P. R. China.
College of Animal Science, South China Agricultural University, Guangzhou 510640, P. R. China.
Poult Sci. 2019 Sep 1;98(9):3705-3714. doi: 10.3382/ps/pez058.
The study investigated the effects of dietary curcumin supplementation on tissue distribution of curcumin and its metabolites, intestinal antioxidant capacity, and expression of detoxification-related genes in ducks. A total of 720 one-day-old male Cherry Valley Pekin ducklings (initial BW 58.6 ± 0.1 g) were randomly assigned to 4 dietary groups each with 6 replicates of 30 ducks using a single factorial arrangement design. Ducks in the control group were fed a basal diet and the remainder were fed the basal diet supplemented with 200, 400, or 800 mg/kg curcumin. The experiment lasted for 21 D. Curcumin was present at 13.12 to 16.18 mg/g in the cecal digesta, 75.50 to 575.40 μg/g in jejunal mucosa, 35.10 to 73.65 μg/g in liver, and 7.02 to 7.88 μg/mL in plasma. The jejunal and hepatic contents of curcumin increased significantly (P < 0.05) in response to supplementation with 400 and 800 mg/kg of curcumin respectively, compared with 200 mg curcumin/kg group. There was a linear (P < 0.001) effect of dietary curcumin on relative abundance of SOD1, GPX1, CAT, HO-1, and Nrf2 transcripts, and a quadratic (P < 0.001) increase in the activities of GSH-Px and T-AOC in jejunal mucosa. The expression of CYP1A4, CYP2D17 increased and CYP1B1, CYP2A6 decreased linearly (P < 0.001) with dietary curcumin concentrations. In addition, dietary curcumin increased gene expression of GST, MRP6, and ABCB1 in jejunal mucosa. In conclusion, dietary supplementation with 200 to 800 mg/kg curcumin enhanced the accumulation of curcumin and its metabolites in jejunum as well as increasing the antioxidant capacity and detoxification potential, which play major roles in the protection of duck intestines against damage.
本研究旨在探讨饲粮中添加姜黄素对姜黄素及其代谢物在组织中的分布、肠道抗氧化能力以及解毒相关基因表达的影响。选用 720 只 1 日龄雄性樱桃谷肉鸭（初始体重 58.6±0.1g），随机分为 4 个处理组，每个处理组 6 个重复，每个重复 30 只鸭，采用单因子试验设计。对照组饲喂基础饲粮，其余 3 组分别在基础饲粮中添加 200、400 和 800mg/kg 姜黄素。试验期为 21d。盲肠内容物中姜黄素含量为 13.1216.18mg/g，空肠黏膜中姜黄素含量为 75.50575.40μg/g，肝脏中姜黄素含量为 35.1073.65μg/g，血浆中姜黄素含量为 7.027.88μg/mL。与 200mg/kg 姜黄素组相比，400 和 800mg/kg 姜黄素组空肠和肝脏中姜黄素含量显著增加（P＜0.05）。饲粮姜黄素对 SOD1、GPX1、CAT、HO-1 和 Nrf2 转录物的相对丰度有线性影响（P＜0.001），对空肠黏膜中 GSH-Px 和 T-AOC 活性有二次影响（P＜0.001）。CYP1A4 和 CYP2D17 的表达量随饲粮姜黄素浓度的增加而线性增加（P＜0.001），CYP1B1 和 CYP2A6 的表达量线性下降（P＜0.001）。此外，饲粮姜黄素增加了空肠黏膜中 GST、MRP6 和 ABCB1 的基因表达。综上所述，饲粮中添加 200~800mg/kg 姜黄素可增加姜黄素及其代谢物在空肠中的积累，提高抗氧化能力和解毒潜力，从而保护鸭肠道免受损伤。
