Douglas Stephens Surgical Research Unit, Murdoch Children's Research Institute, Melbourne, Australia.
Douglas Stephens Surgical Research Unit, Murdoch Children's Research Institute, Melbourne, Australia; School of Basic Medical Science, Inner Mongolia Medical University, Jinshan Street, Jinshan Development Zone Huhhot, Inner Mongolia 010110, PR China.
J Pediatr Surg. 2020 Sep;55(9):1952-1958. doi: 10.1016/j.jpedsurg.2019.12.019. Epub 2020 Jan 7.
The inguinoscrotal stage of testicular descent is characterized by an increase in cell density and collagen fibers as the gubernaculum undergoes cell division and increases Extracellular Matrix (ECM) activity. Rats that lack the enzyme Adamts16, a known ECM proteinase, develop cryptorchidism postnatally and are infertile. Therefore, this study aims to investigate the link between the Adamts16 enzyme and congenital undescended testes (UDT) in Adamts16 knockout (KO) rats during postnatal development.
Formalin-fixed specimens of Wild-Type, Adamts16 heterozygous and Adamts16 homozygous KO rats post birth were sectioned and used for standard H&E histology and Masson's trichrome staining. A quantitative analysis on image J was performed to determine the intensity of collagen fibers within the inguinoscrotal fat pad (IFP) (n = 3 age/genotype).
The migration of the gubernaculum within the Adamts16 heterozygous and Adamts16 KO rat was considerably disrupted. Furthermore, the Masson's trichrome staining demonstrated a significant increase in collagen fibers around the gubernaculum of rats that lacked Adamts16 enzyme at day 8.
This study reports a failure of gubernacular migration leading to UDT in Adamts16 KO rats during development, suggesting that the expression of Adamts16 gene is critical for normal gubernacular migration through the breakdown of collagen fibers within the IFP.
睾丸下降至腹股沟阴囊的阶段的特征是细胞密度和胶原纤维增加，因为提睾肌经历细胞分裂并增加细胞外基质（ECM）活性。缺乏酶 Adamts16 的大鼠在出生后会发生隐睾症并且不育，Adamts16 是一种已知的 ECM 蛋白酶。因此，本研究旨在探讨 Adamts16 敲除（KO）大鼠在出生后发育过程中 Adamts16 酶与先天性未降睾丸（UDT）之间的联系。
对出生后野生型、Adamts16 杂合子和 Adamts16 纯合子 KO 大鼠的福尔马林固定标本进行切片，用于标准 H&E 组织学和 Masson 三色染色。使用 Image J 对图像进行定量分析，以确定腹股沟阴囊脂肪垫（IFP）内胶原纤维的强度（n = 3 个年龄/基因型）。
Adamts16 杂合子和 Adamts16 KO 大鼠的提睾肌迁移明显受到干扰。此外，Masson 三色染色显示，在缺乏 Adamts16 酶的大鼠中，第 8 天的 gubernaculum 周围的胶原纤维明显增加。
本研究报告了 Adamts16 KO 大鼠在发育过程中 gubernacular 迁移失败导致 UDT，表明 Adamts16 基因的表达对于通过 IFP 内胶原纤维的分解正常 gubernacular 迁移至关重要。
