Chen Xianyan, Liu Ben, Li Ying, Han Le, Tang Xin, Deng Wenjia, Lai Wei, Wan Miaojian
Department of Dermatology, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, China.
Department of Dermatology, Hexian Medical Affiliated Hospital of Southern Medical University, Guangzhou, China.
Front Pharmacol. 2020 Jan 23;10:1528. doi: 10.3389/fphar.2019.01528. eCollection 2019.
Dihydrotestosterone (DHT) is the most potent androgen that regulates hair cycling. Hair cycling involves cross-talk between the androgen and Wnt/β-catenin pathways. However, how DHT regulates hair follicle (HF) growth through the Wnt/β-catenin pathway has not been well investigated. This study aimed to investigate the roles of DHT in hair growth and . Human scalp HFs were treated with different concentrations of DHT (10, 10, 10, 10, and 10 mol/L) for 10 days. The effects of DHT on hair shaft elongation, the proliferation of hair matrix cells, and the levels of β-catenin, GSK-3β, and phosphorylated GSK-3β (ser9) were evaluated in the cultured HFs. The effects of DHT were further investigated in C57BL/6 mice. Moreover, the growth of cultured human HFs was observed after interfering with the β-catenin pathway through inhibitors or activators in the presence or absence of DHT. We found that different concentrations of DHT had different effects on human HFs and C57BL/6 mice. At 10 mol/L, DHT inhibited HF growth and β-catenin/p-GSK-3β expression, whereas 10 mol/L DHT induced HF growth and β-catenin/p-GSK-3β expression. In addition, a β-catenin inhibitor (21H7) inhibited HF growth , while a β-catenin activator (IM12) promoted HF growth and antagonized the inhibition of HFs by high levels of DHT. These results suggest that DHT plays a pivotal role in region-specific hair growth, which may be related to the Wnt/β-catenin pathway.
双氢睾酮（DHT）是调节毛发周期的最具活性的雄激素。毛发周期涉及雄激素与Wnt/β-连环蛋白信号通路之间的相互作用。然而，DHT如何通过Wnt/β-连环蛋白信号通路调节毛囊（HF）生长尚未得到充分研究。本研究旨在探讨DHT在毛发生长中的作用。将不同浓度（10⁻⁹、10⁻⁸、10⁻⁷、10⁻⁶和10⁻⁵mol/L）的DHT作用于人头皮毛囊10天。在培养的毛囊中评估DHT对毛干伸长、毛基质细胞增殖以及β-连环蛋白、糖原合成酶激酶-3β（GSK-3β）和磷酸化GSK-3β（ser9）水平的影响。在C57BL/6小鼠中进一步研究DHT的作用。此外，在存在或不存在DHT的情况下，通过抑制剂或激活剂干扰β-连环蛋白信号通路后，观察培养的人毛囊的生长情况。我们发现不同浓度的DHT对人毛囊和C57BL/6小鼠有不同影响。在10⁻⁵mol/L时，DHT抑制毛囊生长以及β-连环蛋白/p-GSK-3β表达，而10⁻⁹mol/L DHT诱导毛囊生长以及β-连环蛋白/p-GSK-3β表达。此外，β-连环蛋白抑制剂（21H7）抑制毛囊生长，而β-连环蛋白激活剂（IM12）促进毛囊生长并拮抗高水平DHT对毛囊的抑制作用。这些结果表明，DHT在区域特异性毛发生长中起关键作用，这可能与Wnt/β-连环蛋白信号通路有关。
