College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China.
Food Funct. 2015 Jul;6(7):2396-404. doi: 10.1039/c5fo00273g.
Both adipocyte hypertrophy and hyperplasia lead to obesity. Here, we isolated cerebrosides from the sea cucumber Cucumaria frondosa (CFC) and examined its anti-adipogenic activity in vitro. CFC inhibited the lipid accumulation of 3T3-L1 cells and suppressed PPARγ and C/EBPα expressions, proving its anti-adipogenic activity. Furthermore, CFC suppressed lipogenesis in mature adipocytes. The WNT/β-catenin pathway acts as an anti-adipogenic factor. CFC enhanced β-catenin expression, promoted its nuclear translocation and up-regulated the expression of CCND1 and c-myc, two target genes of β-catenin. Moreover, after cells were treated with the β-catenin inhibitor 21H7, β-catenin nuclear translocation and transcription activity can be recovered by CFC. These findings suggested that CFC promoted the activation of the WNT/β-catenin pathway. Besides, CFC enhanced the expressions of Fz1, LRP5 and LRP6, while it had no effect on the expressions of Wnt10b and GSK3β. These findings indicated that CFC exhibits anti-adipogenic activity through enhancing the activation of the WNT/β-catenin pathway, which was mediated by FZ and LRPs.
脂肪细胞肥大和增生都会导致肥胖。在这里，我们从海参 Cucumaria frondosa (CFC) 中分离出脑苷脂，并在体外研究其抗脂肪生成活性。CFC 抑制 3T3-L1 细胞的脂质积累，并抑制 PPARγ 和 C/EBPα 的表达，证明其具有抗脂肪生成活性。此外，CFC 抑制成熟脂肪细胞中的脂肪生成。WNT/β-catenin 途径作为一种抗脂肪生成因子。CFC 增强β-catenin 的表达，促进其核转位，并上调两个β-catenin 靶基因 CCND1 和 c-myc 的表达。此外，在用β-catenin 抑制剂 21H7 处理细胞后，CFC 可恢复β-catenin 的核转位和转录活性。这些发现表明 CFC 促进了 WNT/β-catenin 途径的激活。此外，CFC 增强了 Fz1、LRP5 和 LRP6 的表达，而对 Wnt10b 和 GSK3β 的表达没有影响。这些发现表明，CFC 通过增强 WNT/β-catenin 途径的激活来发挥抗脂肪生成活性，这是由 FZ 和 LRPs 介导的。
