Zhu Manli, He Youwei, Li Yi, Ren Tirong, Liu Hao, Huang Junbin, Jiang Daohong, Hsiang Tom, Zheng Lu
The Key Lab of Plant Pathology of Hubei Province, Huazhong Agricultural University, Wuhan, China.
School of Environmental Sciences, University of Guelph, Guelph, ON, Canada.
Front Microbiol. 2020 Jan 21;10:3099. doi: 10.3389/fmicb.2019.03099. eCollection 2019.
Clubroot disease caused by can lead to serious yield losses in crucifers such as . In this study, 323 bacterial strains were isolated from the rhizosphere of severely diseased in Dangyang county, Hubei province, China. Antagonistic strains were first identified based on dual culture inhibition zones with and . These were then further screened in germination inhibition and viability assays of resting spores of . Finally, eight of the antagonistic strains were found to significantly reduce the disease severity of clubroot by more than 40% under greenhouse conditions, and two strains, F85 and T113, were found to have efficacy of more than 80%. Root hair infection experiments showed that F85 and T113 can inhibit early infection of root hairs, reduce the differentiation of primary plasmodia of , and inhibit formation of secondary zoosporangia. Based on sequence analysis of 16S rDNA gene, gene and 22 housekeeping genes as well as carbon source utilization analysis, the F85 was identified as and T113 as . Genome analysis, PCR and RT-PCR detection revealed that both F85 and T113 harbor various antibiotic biosynthesis gene clusters required to form peptides with antimicrobial activity. To our knowledge, this is the first report of as a biocontrol agent against clubroot disease.
由[病原体名称未给出]引起的根肿病会导致十字花科植物如[植物名称未给出]严重减产。在本研究中，从中国湖北省当阳县重病[植物名称未给出]的根际分离出323株细菌菌株。首先基于与[对照菌株未给出]和[对照菌株未给出]的对峙培养抑菌圈鉴定出拮抗菌株。然后在[休眠孢子未给出]的萌发抑制和活力测定中对这些菌株进行进一步筛选。最后，发现其中8株拮抗菌株在温室条件下可使根肿病的病情严重程度显著降低40%以上，F85和T113两株菌株的防治效果超过80%。根毛感染实验表明，F85和T113可抑制根毛的早期感染，减少[病原菌名称未给出]原生质团的分化，并抑制次生游动孢子囊的形成。基于16S rDNA基因、[基因名称未给出]基因和22个管家基因的序列分析以及碳源利用分析，F85被鉴定为[细菌名称未给出]，T113被鉴定为[细菌名称未给出]。基因组分析、PCR和RT-PCR检测表明，F85和T113均含有形成具有抗菌活性肽所需的各种抗生素生物合成基因簇。据我们所知，这是关于[细菌名称未给出]作为防治根肿病生防菌的首次报道。
