Institute of Horticulture, Henan Academy of Agricultural Sciences, Graduate T&R Base of Zhengzhou University, Zhengzhou 450002, China.
Henan International Joint Laboratory of Crop Gene Resources and Improvement, School of Agricultural Sciences, Zhengzhou University, Zhengzhou 450001, China.
Int J Mol Sci. 2023 Jan 26;24(3):2414. doi: 10.3390/ijms24032414.
Clubroot is an infectious root disease caused by in crops, which can cause immeasurable losses. We analyzed integrative transcriptome, small RNAs, degradome, and phytohormone comprehensively to explore the infection mechanism of In this study, root samples of resistant line material BrT24 (R-line) and susceptible line material Y510-9 (S-line) were collected at four different time points for cytological, transcriptome, miRNA, and degradome analyses. We found the critical period of disease resistance and infection were at 0-3 DAI (days after inoculation) and 9-20 DAI, respectively. Based on our finding, we further analyzed the data of 9 DAI vs. 20 DAI of S-line and predicted the key genes , , , , , and , which were related to clubroot disease development and regulating disease resistance mechanisms. These genes are mainly related to auxin, cytokinin, jasmonic acid, and ethylene cycles. We proposed a regulatory model of plant hormones under the mRNA-miRNA regulation in the critical period of infection by using the present data of the integrative transcriptome, small RNAs, degradome, and phytohormone with our previously published results. Our integrative analysis provided new insights into the regulation relationship of miRNAs and plant hormones during the process of disease infection with
根肿病是一种由 引起的传染性根部疾病，可导致不可估量的损失。我们综合分析了转录组、小 RNA、降解组和植物激素，以探索 感染的机制。在这项研究中，我们收集了抗材料 BrT24（R 线）和感材料 Y510-9（S 线）在四个不同时间点的根样进行细胞学、转录组、miRNA 和降解组分析。我们发现抗病和感染的关键时期分别在接种后 0-3 天（DAI）和 9-20 DAI。基于我们的发现，我们进一步分析了 S 线 9 DAI 与 20 DAI 的数据，并预测了关键基因 、 、 、 、 、 ，这些基因与根肿病的发展和调节抗病机制有关。这些基因主要与生长素、细胞分裂素、茉莉酸和乙烯循环有关。我们利用综合转录组、小 RNA、降解组和植物激素的现有数据以及我们之前发表的结果，提出了在 感染的关键时期，mRNA-miRNA 调控下植物激素的调控模型。我们的综合分析为疾病感染过程中 miRNA 和植物激素的调控关系提供了新的见解。
