Huang Gan, Han Meixiang, Jian Lin, Chen Yanbo, Sun Shulan, Wang Xiaojing, Wang Yaqin
Guangdong Provincial Key Laboratory of Biotechnology for Plant Development School of Life Sciences South China Normal University, Guangzhou, China.
Front Plant Sci. 2020 Jan 24;10:1737. doi: 10.3389/fpls.2019.01737. eCollection 2019.
Petal morphogenesis has a profound influence on the quality of ornamental flowers. Most current research on petal development focuses on the early developmental stage, and little is known about the late developmental stage. Previously, it was reported that the gene [a gerbera homolog of the gibberellin-stimulated transcript 1 () from tomato] negatively regulates ray petal growth during the late stage of development by inhibiting longitudinal cell expansion. To explore the molecular mechanisms of the role of in petal growth inhibition, an ethylene insensitive 3-like 1 (EIL1) protein was identified from a cDNA library by yeast one-hybrid screening. Direct binding between GhEIL1 and the promoter was confirmed by electrophoretic mobility shift and dual-luciferase assays. The expression profiles of and were correlated during petal development, while a transient transformation assay suggested that GhEIL1 regulates expression and may be involved in the inhibition of ray petal elongation and cell elongation. To study the effect of ethylene on ray petal growth, a hormone treatment assay was performed in detached ray petals. The results showed that petal elongation is limited and promoted by ACC and 1-MCP, respectively, and the expression of and is regulated and coordinated during this process. Taken together, our research suggests that GhEIL1 forms part of the ethylene signaling pathway and activates to regulate ray petal growth during the late developmental stage in .
花瓣形态发生对观赏花卉的品质有深远影响。目前大多数关于花瓣发育的研究集中在早期发育阶段，而对后期发育阶段了解甚少。此前有报道称，基因[番茄赤霉素刺激转录本1（）的非洲菊同源基因]在发育后期通过抑制纵向细胞扩展来负调控舌状花瓣的生长。为了探究在花瓣生长抑制中作用的分子机制，通过酵母单杂交筛选从一个cDNA文库中鉴定出一种乙烯不敏感3样1（EIL1）蛋白。通过电泳迁移率变动分析和双荧光素酶测定证实了GhEIL1与启动子之间的直接结合。在花瓣发育过程中，和的表达谱相关，而瞬时转化试验表明GhEIL1调节的表达，并可能参与舌状花瓣伸长和细胞伸长抑制。为了研究乙烯对舌状花瓣生长的影响，对离体舌状花瓣进行了激素处理试验。结果表明，花瓣伸长分别受到ACC和1-MCP的限制和促进，并且在此过程中和的表达受到调节和协调。综上所述，我们的研究表明GhEIL1构成乙烯信号通路的一部分，并激活以调节非洲菊发育后期舌状花瓣的生长。
