Zhao Zheng Lin, Zhao Guang Wen, Li Li, Li Meng Quan, Guan Li Xin, Yang Xu Dong, Li Hou Zhong, Lin Feng, Lee Jong Rok, Zhao Rong Jie
13Department of Biochemistry, Mudanjiang Medical University, Mudanjiang, 157011 P.R. China.
23Department of General Surgery, Yanbian Medical College, Yanbian University, Yanji, 133000 China.
Toxicol Res. 2009 Mar;25(1):17-21. doi: 10.5487/TR.2009.25.1.017. Epub 2009 Mar 1.
The effects of aqueous extract of (AESC) on cadmium-induced changes of monoamine neurotransmitters in the different brain regions of adult rats were investigated. Male rats were received intraperitoneal (i.p.) administration of CdCl (0.6 mg/kg/d) for 21 days and sacrificed 7 days after the last administration. Concentrations of norepinephrine (NE), dopamine (DA) in striatum and serotonin (5-HT), 5-hydroxyindole acetic acid (5-HIAA) in cortex were measured by HPLC. There were significant decreases of NE, DA, 5-HT and 5-HIAA in Cd intoxicated rats ( < 0.05), while pretreatment with AESC (20 mg/kg/d or 60 mg/kg/d, p.o., 30 min before CdCl) greatly inhibited the decrease of monoamine transmitters, respectively ( < 0.05). Also, AESC significantly increased the reduction of glutathione contents and superoxide dismutase activities in cortex induced by CdCl. These results suggest that AESC ameliorates Cd-induced depletion of monoamine neu-rotransmitters in brain through its antioxidant activity.
研究了七叶树提取物（AESC）对成年大鼠不同脑区镉诱导的单胺类神经递质变化的影响。雄性大鼠腹腔注射CdCl₂（0.6 mg/kg/d），持续21天，并在末次给药7天后处死。采用高效液相色谱法测定纹状体中去甲肾上腺素（NE）、多巴胺（DA）以及皮质中5-羟色胺（5-HT）、5-羟吲哚乙酸（5-HIAA）的浓度。镉中毒大鼠的NE、DA、5-HT和5-HIAA显著降低（P<0.05），而用AESC（20 mg/kg/d或60 mg/kg/d，口服，在CdCl₂前30分钟给药）预处理可分别显著抑制单胺类递质的降低（P<0.05）。此外，AESC显著增加了CdCl₂诱导的皮质中谷胱甘肽含量降低和超氧化物歧化酶活性降低。这些结果表明，AESC通过其抗氧化活性改善了镉诱导的脑中单胺类神经递质耗竭。
