Park Eun-Jung, Park Young-Kwon, Park Kwangsik
13College of Pharmacy, Dongduk Women's University, 23-1, Wolgok-dong, Seongbuk-gu, Seoul, 136-714 Korea.
23Faculty of Environmental Engineering, College of Urban Science, University of Seoul, 90 Jeonnong-dong, Dongdaemun-gu, Seoul, 130-743 Korea.
Toxicol Res. 2009 Jun;25(2):79-84. doi: 10.5487/TR.2009.25.2.079. Epub 2009 Jun 1.
Cerium oxide nanoparticles (size: 30 nm) were prepared by the supercritical synthesis method, Acute oral toxicity and tissue distribution of the nanoparticles were evaluated by a single administration in rats. Oral administration of the nanoparticles to the rats did not lead to death when the animals were treated by a dose of 5 g/kg (high dose) as well as 100 mg/kg (low dose). Abnormal clinical signs, changes in serum biochemistry and hematology were not observed in high-dose treated group compared to the vehicle control group. Lesions in liver, lung and kidney were not observed in high-dose treated group by histopathological examination. Tissue distribution analysis in liver, kidney, spleen, lung, testis and brain was performed on day 1, day 7 and day 14 after treatment. The average values of the accumulated cerium oxide nanoparticles were elevated in all tissues but statistical significance was only shown in lung. Low levels of tissue distributions after a single oral administration seem to be the low bioavailability of the nanoparticles.
采用超临界合成法制备了氧化铈纳米颗粒（尺寸：30 nm），通过对大鼠单次给药评估了纳米颗粒的急性经口毒性和组织分布。当以5 g/kg（高剂量）和100 mg/kg（低剂量）对大鼠进行口服纳米颗粒处理时，动物未出现死亡。与赋形剂对照组相比，高剂量处理组未观察到异常临床体征、血清生化和血液学变化。通过组织病理学检查，高剂量处理组未观察到肝脏、肺和肾脏的病变。在处理后的第1天、第7天和第14天对肝脏、肾脏、脾脏、肺、睾丸和大脑进行了组织分布分析。所有组织中氧化铈纳米颗粒的累积平均值均升高，但仅在肺中显示出统计学意义。单次口服给药后组织分布水平较低似乎是纳米颗粒生物利用度较低的原因。
