Department of Predictive Toxicology, Korea Institute of Toxicology, 141 Gajeong-ro, Yuseong-gu, Daejeon, 34114, Republic of Korea.
Department of Human and Environmental Toxicology, University of Science & Technology, Daejeon, 34113, Republic of Korea.
Sci Rep. 2024 Mar 7;14(1):5657. doi: 10.1038/s41598-024-54659-9.
Cerium oxide nanoparticles (CeO NPs, NM-212) are well-known for their catalytic properties and antioxidant potential, and have many applications in various industries, drug delivery, and cosmetic formulations. CeO NPs exhibit strong antimicrobial activity and can be used to efficiently remove pathogens from different environments. However, knowledge of the toxicological evaluation of CeO NPs is too limited to support their safe use. In this study, CeO NPs were orally administered to Sprague Dawley rats for 13 weeks at the doses of 0, 10, 100, and 1000 mg/kg bw/day, followed by a four week recovery period. The hematology values for the absolute and relative reticulocyte counts in male rats treated with 1000 mg/kg bw/day CeO NPs were lower than those in control rats. The clinical chemistry values for sodium and chloride in the treated male rat groups (100 and 1000 mg/kg/day) and total protein and calcium in the treated female rat groups (100 mg/kg/day) were higher than those in the control groups. However, these changes were not consistent in both sexes, and no abnormalities were found in the corresponding pathological findings. The results showed no adverse effects on any of the parameters assessed. CeO NPs accumulated in the jejunum, colon, and stomach wall of rats administered 1000 mg/kg CeO NPs for 90 days. However, these changes were not abnormal in the corresponding histopathological and immunohistochemical examinations. Therefore, 1000 mg/kg bw/day may be considered the "no observed adverse effect level" of CeO NPs (NM-212) in male and female SD rats under the present experimental conditions.
氧化铈纳米颗粒（CeO NPs，NM-212）以其催化特性和抗氧化潜力而闻名，在许多行业、药物输送和化妆品配方中有广泛的应用。CeO NPs 表现出很强的抗菌活性，可用于有效地从不同环境中去除病原体。然而，对 CeO NPs 的毒理学评价的了解还很有限，无法支持其安全使用。在这项研究中，CeO NPs 以 0、10、100 和 1000mg/kg bw/day 的剂量经口给予 Sprague Dawley 大鼠 13 周，随后进行为期 4 周的恢复期。用 1000mg/kg bw/day CeO NPs 处理的雄性大鼠的绝对和相对网织红细胞计数的血液学值低于对照组大鼠。处理雄性大鼠组（100 和 1000mg/kg/day）的血清钠和氯以及处理雌性大鼠组（100mg/kg/day）的总蛋白和钙的临床化学值高于对照组。然而，这些变化在两性中并不一致，在相应的病理发现中没有发现异常。结果表明，在所评估的参数中没有任何不良影响。CeO NPs 在给予 1000mg/kg CeO NPs 90 天的大鼠的空肠、结肠和胃壁中积累。然而，在相应的组织病理学和免疫组织化学检查中，这些变化并不异常。因此，在目前的实验条件下，1000mg/kg bw/day 可被认为是 CeO NPs（NM-212）在雄性和雌性 SD 大鼠中的“无观察到不良效应水平”。
