Evaluation of Antioxidative Activity of Leaves on Non-lipid Oxidative Damage.

Present study was conducted to evaluate the antioxidative activity of the leaves on non-lipid oxidative damage. The antioxidative activity of methanolic (MeOH) extract of the leaves on non-lipid oxidation, including liposome oxidation, deoxyribose oxidation, protein oxidation, chelating activity against metal ions, scavenging activity against hydrogen peroxide, scavenging activity against hydroxyl radical and 2'-deoxyguanosine (2'-dG) oxidation were investigated. The MeOH extract of the leaves exhibited high antioxidative activity in the liposome model system. Deoxyribose peroxidation was inhibited by the MeOH extract of the leaves and MeOH extract of the leaves provided remarkable protection against damage to deoxyribose. Protective effect of MeOH extracts of the leaves on protein damage was observed at 600 µg level (82.05%). The MeOH extracts of the leaves at 300 µg revealed metal binding ability (32.64%) for hydrogen peroxide. Furthermore, the oxidation of 2'-deoxyguanosine (2'-dG) to 8-hydroxy-2-deoxyguanosine (8-OH-2'dG) was inhibited by MeOH extracts of the leaves and scavenging activity for hydroxyl radical exhibited a remarkable effect. From the results in the present study on biological model systems, we concluded that MeOH extract of the leaves was effective in the protection of non-lipids against various oxidative model systems.