Hah Dae-Sik, Kim Chung-Hui, Kim Euikyung, Kim Jong-Shu
Gyeongnam Livestock Promotion Institute Middle-branch, Changwon, 541-703 Korea.
212Department of Animal Science and Biotechnology, Jinju National University, Jinju, 660-758 Korea.
Toxicol Res. 2009 Dec;25(4):243-251. doi: 10.5487/TR.2009.25.4.243. Epub 2009 Dec 30.
Present study was conducted to evaluate the antioxidative activity of the leaves on non-lipid oxidative damage. The antioxidative activity of methanolic (MeOH) extract of the leaves on non-lipid oxidation, including liposome oxidation, deoxyribose oxidation, protein oxidation, chelating activity against metal ions, scavenging activity against hydrogen peroxide, scavenging activity against hydroxyl radical and 2'-deoxyguanosine (2'-dG) oxidation were investigated. The MeOH extract of the leaves exhibited high antioxidative activity in the liposome model system. Deoxyribose peroxidation was inhibited by the MeOH extract of the leaves and MeOH extract of the leaves provided remarkable protection against damage to deoxyribose. Protective effect of MeOH extracts of the leaves on protein damage was observed at 600 µg level (82.05%). The MeOH extracts of the leaves at 300 µg revealed metal binding ability (32.64%) for hydrogen peroxide. Furthermore, the oxidation of 2'-deoxyguanosine (2'-dG) to 8-hydroxy-2-deoxyguanosine (8-OH-2'dG) was inhibited by MeOH extracts of the leaves and scavenging activity for hydroxyl radical exhibited a remarkable effect. From the results in the present study on biological model systems, we concluded that MeOH extract of the leaves was effective in the protection of non-lipids against various oxidative model systems.
本研究旨在评估[植物名称]叶片对非脂质氧化损伤的抗氧化活性。研究了[植物名称]叶片甲醇提取物（MeOH）在非脂质氧化方面的抗氧化活性，包括脂质体氧化、脱氧核糖氧化、蛋白质氧化、对金属离子的螯合活性、对过氧化氢的清除活性、对羟基自由基的清除活性以及2'-脱氧鸟苷（2'-dG）氧化。[植物名称]叶片的MeOH提取物在脂质体模型系统中表现出较高的抗氧化活性。[植物名称]叶片的MeOH提取物抑制了脱氧核糖过氧化，并且[植物名称]叶片的MeOH提取物对脱氧核糖损伤提供了显著的保护。在600μg水平观察到[植物名称]叶片的MeOH提取物对蛋白质损伤的保护作用（82.05%）。300μg的[植物名称]叶片的MeOH提取物显示出对过氧化氢的金属结合能力（32.64%）。此外，[植物名称]叶片的MeOH提取物抑制了2'-脱氧鸟苷（2'-dG）氧化为8-羟基-2'-脱氧鸟苷（8-OH-2'dG），并且对羟基自由基的清除活性表现出显著效果。从本研究在生物模型系统中的结果来看，我们得出结论，[植物名称]叶片的MeOH提取物在保护非脂质免受各种氧化模型系统损伤方面是有效的。
