Key Laboratory of Soil Microbiology, Ministry of Agriculture, Department of Microbiology and Immunology, College of Biological Sciences, China Agricultural University, Beijing 100193, PR China.
State Key Laboratory of Biocontrol and Guangdong Key Laboratory of Plant Resources, College of Ecology and Evolution, Sun Yat-Sen University, Guangzhou, 510275, PR China.
Int J Syst Evol Microbiol. 2020 Apr;70(4):2312-2317. doi: 10.1099/ijsem.0.004036. Epub 2020 Feb 10.
A Gram-stain-negative, non-motile and ovoid bacterial strain, designated 4-2, was isolated from oil-contaminated water which was collected from Xinjiang Province, north-west PR China. The 16S rRNA gene sequence analysis showed that strain 4-2 belonged to the genus . The species with highest similarity to strain 4-2 was YIM 90738 (97.83 %), followed by '' M26 (97.83 %) and SYSUP0003 (97.25 %). The average nucleotide identity values between 4-2 and three type strains were 84.69, 77.88 and 74.07 %, respectively. The genomic DNA G+C content of strain 4-2 was 61.4 mol%. Chemotaxonomical characteristic results showed that the respiratory quinone was ubiquinone Q-10 and the major fatty acids were summed feature 8 (C 7 or C 6) and C cyclo 8. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, unidentified phospholipids, an unidentified aminolipid and an unidentified polar lipid. The predominant polyamines were putrescine, cadaverine and spermidine. On the basis of phenotypic, chemotaxonomic and phylogenetic inferences, strain 4-2 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is 4-2 (=CGMCC 1.13669=LMG 30882).
一株革兰氏阴性、非运动和卵圆形的细菌菌株,被命名为 4-2,从中国西北部新疆省的污染水中分离出来。16S rRNA 基因序列分析表明,菌株 4-2 属于 属。与菌株 4-2 最相似的物种是 YIM 90738(97.83%),其次是 '' M26(97.83%)和 SYSUP0003(97.25%)。4-2 与三株模式株之间的平均核苷酸同一性值分别为 84.69%、77.88%和 74.07%。菌株 4-2 的基因组 DNA G+C 含量为 61.4mol%。化学分类学特征表明,呼吸醌是泛醌 Q-10,主要脂肪酸是 summed feature 8(C 7 或 C 6)和 C 环 8。极性脂谱由双磷脂酰甘油、磷脂酰甘油、磷脂酰胆碱、未鉴定的磷脂、未鉴定的氨基脂和未鉴定的极性脂组成。主要的多胺是腐胺、尸胺和亚精胺。基于表型、化学分类和系统发育推断,菌株 4-2 代表了属的一个新种,建议将其命名为 sp. nov.。模式株为 4-2(=CGMCC 1.13669=LMG 30882)。