[大鼠骨骼肌中辅酶NADH和FAD的荧光强度随死后时间间隔的测定]
[Determination of the fluorescence intensity of coenzymes NADH and FAD in the skeletal muscle of the rat depending on the post-mortem interval].
作者信息
Babkina A S, Sundukov D V, Golubev A M, Ryzhkov I A, Tsokolaeva Z I, Zarzhetsky Yu V
机构信息
Department of Forensic Medicine, Medical Institute of the Peoples' Friendship University of Russia, Moscow, Russia, 117198; General resuscitation Research Center of the V.A. Negovsky Federal Scientific and Clinical Center of resuscitation and rehabilitation, Moscow, Russia, 107031.
Department of Forensic Medicine, Medical Institute of the Peoples' Friendship University of Russia, Moscow, Russia, 117198.
出版信息
Sud Med Ekspert. 2020;63(1):31-35. doi: 10.17116/sudmed20206301131.
Aim of the study is to identify patterns of variations of the fluorescence intensity of NADH (reduced nicotinamide adenine dinucleotide) and FAD (oxidized flavin adenine dinucleotide) in the skeletal muscle depending on the time since death. For the evaluation of fluorescence intensity of the studied coenzymes, laser-induced spectroscopy in situ was used. We revealed the dynamic of the fluorescence intensity of NADH and FAD in the skeletal muscle of a rat at different times during the post-mortem period, and theoretically justified the observed phenomena. The results obtained allow us to consider the studied indicators as a potential criterion for determining the post-mortem interval.
本研究的目的是确定骨骼肌中烟酰胺腺嘌呤二核苷酸（NADH）和黄素腺嘌呤二核苷酸（FAD）的荧光强度随死亡时间的变化模式。为了评估所研究辅酶的荧光强度，采用了原位激光诱导光谱法。我们揭示了大鼠骨骼肌在死后不同时间点NADH和FAD荧光强度的动态变化，并从理论上对观察到的现象进行了论证。所得结果使我们能够将所研究的指标视为确定死后间隔时间的潜在标准。
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