Huang Sheng-Po, Chuang Yuan-Jhe, Lee Wen-Bin, Tsai Yi-Cheng, Lin Chang-Ni, Hsu Keng-Fu, Lee Gwo-Bin
Institute of NanoEngineering and MicroSystems, National Tsing Hua University, Hsinchu, Taiwan.
Department of Obstetrics and Gynecology, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan, 70403 Taiwan.
Lab Chip. 2020 Mar 17;20(6):1103-1109. doi: 10.1039/c9lc00979e.
Accurate cancer diagnostic methods are of urgent need. Since traditional immunohistochemistry (IHC)-based approaches, while reliable, are labor-intensive and require well-trained technicians, we developed an integrated microfluidic platform capable of labeling ovarian cancer biomarkers (i.e. aptamer) within formalin-fixed, paraffin embedded tissues via molecular probes. Both aptamer-based 1) fluorescent staining and 2) IHC staining of clinical tissue samples could be automated in the microfluidic system in only 2-3 h (40-50% faster than conventional approaches) with <0.5 mL of reagents, signifying that this device could serve as a promising diagnostic tool for ovarian cancer.
迫切需要准确的癌症诊断方法。由于基于传统免疫组织化学（IHC）的方法虽然可靠，但劳动强度大且需要训练有素的技术人员，因此我们开发了一种集成微流控平台，该平台能够通过分子探针在福尔马林固定、石蜡包埋的组织中标记卵巢癌生物标志物（即适体）。在微流控系统中，仅需2-3小时（比传统方法快40-50%），使用少于0.5毫升的试剂，就可以自动完成基于适体的临床组织样本的1）荧光染色和2）免疫组织化学染色，这表明该设备有望成为卵巢癌的诊断工具。
