无酶放大表面增强拉曼散射免疫分析用于疾病生物标志物的超灵敏检测。
Enzyme-free amplified SERS immunoassay for the ultrasensitive detection of disease biomarkers.
机构信息
Key Laboratory for Advanced Materials & School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai, 200237, P. R. China.
Aliex Technology Group Co., Ltd, No. 152, Lane 468, North Hengshahe Road, Shanghai, 201108, P. R. China.
出版信息
Chem Commun (Camb). 2020 Mar 5;56(19):2933-2936. doi: 10.1039/c9cc09379f.
We developed a novel enzyme-free amplified SERS immunoassay by combining silver nanoparticle (AgNP)-linked immunoreaction and SERS transduction for the detection of disease biomarkers. As a proof of concept, our method was successfully illustrated with the disease biomarker α-fetoprotein with the detection limit of 3.3 × 10-13 g mL-1 and a double-blind experiment consisting of tens of serum samples was performed to confirm its reliability.
我们开发了一种新型的无酶扩增 SERS 免疫分析方法，通过结合银纳米粒子（AgNP）连接的免疫反应和 SERS 转导来检测疾病生物标志物。作为概念验证，我们的方法成功地用疾病生物标志物甲胎蛋白进行了说明，检测限为 3.3×10-13 g mL-1，并且进行了包含数十个血清样本的双盲实验以确认其可靠性。
Zotero 插件