基于 SERS 的免疫分析增强银探针用于阿尔茨海默病生物标志物的选择性分离和检测。
SERS-Based Immunoassay Enhanced with Silver Probe for Selective Separation and Detection of Alzheimer's Disease Biomarkers.
机构信息
Medical Technology School of Xuzhou Medical University, Xuzhou, Jiangsu, 221000, People's Republic of China.
Jiangsu Key Laboratory of Medical Science and Laboratory Medicine, School of Medicine, Jiangsu University, Zhenjiang, Jiangsu, 212013, People's Republic of China.
出版信息
Int J Nanomedicine. 2021 Mar 5;16:1901-1911. doi: 10.2147/IJN.S293042. eCollection 2021.
PURPOSE
Developing a sensitive SERS-based method to quantitatively detect serum biomarkers (Aβ1-42 and P-Tau-181) for the early diagnosis of Alzheimer's disease (AD).
METHODS
In this study, a novel SERS-based sandwich immunoassay, which consists of tannin-capped silver nanoparticles and magnetic graphene oxide (FeO@GOs), was developed. We firstly applied this method for the detection of protein standards in buffer solution, obtaining the regression equation. Then, its potential value on real serum samples of AD was further explored.
RESULTS
The detection linear range of Aβ1-42 and P-Tau-181 protein standards were observed to range from 100 pg mL to 10 fg mL, 100 pg mL to 1 fg mL respectively. We finally explored clinical application of the proposed method in 63 serum samples. As a result, P-tau-181 differentiated AD from non-AD dementia patients (AUC = 0.770), with a more favored ROC than Aβ1-42 (AUC = 0.383).
CONCLUSION
The developed SERS-based immunoassay is successfully applied to the determination of Aβ1-42 and P-Tau-181 in human serum specimens, which provides a promising tool for the early diagnosis of AD.
目的
开发一种基于 SERS 的灵敏方法，用于定量检测血清生物标志物（Aβ1-42 和 P-Tau-181），以实现阿尔茨海默病（AD）的早期诊断。
方法
在这项研究中，开发了一种新型的基于 SERS 的三明治免疫测定法，该方法由单宁封端的银纳米粒子和磁性氧化石墨烯（FeO@GOs）组成。我们首先应用该方法检测缓冲液溶液中的蛋白质标准品，获得回归方程。然后，进一步探索了其在 AD 实际血清样本中的潜在价值。
结果
观察到 Aβ1-42 和 P-Tau-181 蛋白标准品的检测线性范围分别为 100 pg mL 至 10 fg mL、100 pg mL 至 1 fg mL。我们最终在 63 个血清样本中探索了该方法的临床应用。结果表明，P-tau-181 能够区分 AD 和非 AD 痴呆患者（AUC = 0.770），其 ROC 优于 Aβ1-42（AUC = 0.383）。
结论
该研究成功应用基于 SERS 的免疫测定法测定人血清标本中的 Aβ1-42 和 P-Tau-181，为 AD 的早期诊断提供了一种有前途的工具。
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