Kamisato J K, Nowakowski M
Department of Pathology, SUNY Health Science Center, Brooklyn 11203.
Immunopharmacology. 1988 Sep-Oct;16(2):89-96. doi: 10.1016/0162-3109(88)90017-3.
A glycan extracted from Coriolus versicolor (PSK, Krestin) which has antitumor and immunomodulator properties produced marked morphological and biochemical changes when added to cultures of mouse peritoneal macrophages. The cells were more spread and elongated than in control cultures, and these changes were accompanied by alterations in the rate of protein and DNA synthesis. In PSK-treated murine peritoneal macrophages the rate of protein synthesis increased above the level seen in control cultures after two days and reached a level twenty-fold higher than control on day four; this elevated rate of protein synthesis was maintained throughout the seven-day observation period. DNA synthesis was induced after four days in the presence of PSK, and reached a level ten-fold higher than control baseline on day five. This induction of DNA synthesis, however, could not be attributed to a mitogenic activity on lymphocytes. The alterations caused by PSK in macrophage metabolism may be related to the immunomodulating and antitumor activities of PSK in vivo.
从云芝中提取的一种聚糖（PSK，克瘤宁）具有抗肿瘤和免疫调节特性，当添加到小鼠腹腔巨噬细胞培养物中时，会产生明显的形态和生化变化。与对照培养物相比，细胞更加铺展和伸长，这些变化伴随着蛋白质和DNA合成速率的改变。在经PSK处理的小鼠腹腔巨噬细胞中，蛋白质合成速率在两天后高于对照培养物中的水平，并在第四天达到比对照高20倍的水平；在整个七天的观察期内，这种升高的蛋白质合成速率一直维持。在PSK存在的情况下，四天后诱导了DNA合成，并在第五天达到比对照基线高10倍的水平。然而，这种DNA合成的诱导不能归因于对淋巴细胞的促有丝分裂活性。PSK引起的巨噬细胞代谢变化可能与其在体内的免疫调节和抗肿瘤活性有关。
