Ebina T, Murata K
Department of Bacteriology, Tohoku University School of Medicine, Sendai.
Jpn J Cancer Res. 1990 Dec;81(12):1307-13. doi: 10.1111/j.1349-7006.1990.tb02695.x.
The antitumor effect of PSK, a Coriolus preparation, at a distant site was analyzed with the use of a double grafted tumor system in which male BALB/c mice received simultaneous intradermal inoculations of Meth-A tumor in the right (10(6) cells) and the left (2 x 10(5) cells) flanks and were then injected with PSK in the right tumor on the third day thereafter. The antitumor effect of intratumoral administration of PSK in the right tumor on days 3, 4 and 5 was compared with the effect of surgical resection of the right tumor on day 5. Three out of 8 mice given PSK intratumorally became tumor-free whereas no mouse tumor-free in the left flank was found among the surgically resected mice. As regards sinecomitant immunity, tumor inoculation into the right flank followed by intra-tumoral administration of PSK on days 3 and 5 and surgical excision of the primary tumor on day 6 resulted in complete rejection of a tumor challenge in the left flank on day 21. The combination of presurgical intratumoral injections of PSK (more than 2 times) and postoperative oral administration of PSK appeared to be most effective in eradicating secondary tumors. Isolated TILs (tumor-infiltrating lymphocytes), obtained from the right tumor (treated with PSK) and the left tumor on day 10 in the double grafted tumor system were cultured in RPMI1640 with 10% fetal calf serum for 24 h. The culture supernatants were harvested and tested for the presence of chemotactic activity for neutrophils or macrophages. Significant neutrophil chemotactic factor (NCF) and macrophage chemotactic factor (MCF) activities were detected in the culture media from PSK-treated TILs that had been cultured for 24 h. Neither significant neutrophil nor macrophage chemotactic activity was detected in the media from untreated TILs. NCF and MCF activities were also detected in the culture supernatant from PSK-treated tumor tissue on day 6. PSK-induced NCF in the murine tumor was neutralized by treatment with anti-human IL-8 IgG, and might be murine IL-8-like factor. Therefore, neutrophil and macrophage infiltrations of tumors following intratumoral injections of PSK are probably mediated by inductions of IL-8-like factor and MCF.
使用双移植肿瘤系统分析了云芝提取物PSK在远处部位的抗肿瘤作用，该系统中雄性BALB/c小鼠在右侧（10⁶个细胞）和左侧（2×10⁵个细胞）胁腹同时进行皮内接种Meth-A肿瘤，然后在第三天给右侧肿瘤注射PSK。将右侧肿瘤在第3、4和5天瘤内注射PSK的抗肿瘤作用与第5天手术切除右侧肿瘤的作用进行比较。8只瘤内注射PSK的小鼠中有3只无瘤，而手术切除的小鼠中左侧胁腹未发现无瘤小鼠。关于伴随免疫，在右侧胁腹接种肿瘤，然后在第3天和第5天进行瘤内注射PSK，并在第6天手术切除原发肿瘤，导致在第21天左侧胁腹的肿瘤攻击被完全排斥。术前瘤内注射PSK（超过2次）与术后口服PSK的联合似乎在根除继发性肿瘤方面最有效。在双移植肿瘤系统中，于第10天从右侧肿瘤（用PSK处理）和左侧肿瘤获得的分离肿瘤浸润淋巴细胞（TILs）在含10%胎牛血清的RPMI1640中培养24小时。收集培养上清液并检测对中性粒细胞或巨噬细胞的趋化活性。在培养24小时的PSK处理的TILs的培养基中检测到显著的中性粒细胞趋化因子（NCF）和巨噬细胞趋化因子（MCF）活性。在未处理的TILs的培养基中未检测到显著的中性粒细胞或巨噬细胞趋化活性。在第6天PSK处理的肿瘤组织的培养上清液中也检测到NCF和MCF活性。用抗人IL-8 IgG处理可中和PSK在鼠肿瘤中诱导的NCF，其可能是鼠IL-8样因子。因此，瘤内注射PSK后肿瘤的中性粒细胞和巨噬细胞浸润可能是由IL-8样因子和MCF的诱导介导的。
