Kurakata Y, Sakagami H, Sato A, Kikuchi K, Takeda M, Asano K, Sato T
First Department of Biochemistry, School of Medicine, Showa University, Tokyo, Japan.
Anticancer Res. 1991 Sep-Oct;11(5):1767-72.
When mouse resident peritoneal macrophages were cultured with PSK (Krestin), a protein-bound polysaccharide extracted from Coriolus versicolor, they became enlarged and elongated and expressed higher NBT-reducing activity. PSK stimulated the production of differentiation-inducing factor and cytotoxic factor by the mouse macrophage-like cell line J774.1, and iodination (incorporation of radioactive iodine into an acid-insoluble fraction) and interleukin-1-like factor production by human peripheral blood monocytes. Among four different PSK subfractions, the highest molecular weight fraction (MW greater than 200 kD) was the most potent. Natural and chemically modified glucans had little or no activity. The data suggest that some unique structure of the highest molecular weight fraction of PSK directly stimulates the monocytes/macrophages.
当将小鼠腹腔常驻巨噬细胞与从云芝中提取的一种蛋白结合多糖PSK（裂褶菌多糖）一起培养时，它们会变大并伸长，且表现出更高的NBT还原活性。PSK刺激小鼠巨噬细胞样细胞系J774.1产生分化诱导因子和细胞毒性因子，以及刺激人外周血单核细胞的碘化作用（将放射性碘掺入酸不溶性部分）和白细胞介素-1样因子的产生。在四种不同的PSK亚组分中，分子量最高的组分（分子量大于200 kD）活性最强。天然和化学修饰的葡聚糖几乎没有活性。数据表明，PSK分子量最高组分的某些独特结构可直接刺激单核细胞/巨噬细胞。
