Department of Orthopedic Surgery, The Third Hospital of Hebei Medical University, No. 139 Ziqiang Road, Qiaoxi District, Shijiazhuang, 050051, Hebei, People's Republic of China.
Key Laboratory of Biomechanics of Hebei Province, The Third Hospital of Hebei Medical University, Shijiazhuang, 050051, Hebei, People's Republic of China.
Int Orthop. 2020 Jun;44(6):1107-1113. doi: 10.1007/s00264-020-04497-x. Epub 2020 Feb 10.
The purpose of this study was to establish a mouse model of proximal fibular osteotomy (PFO), and to determine if PFO could delay degeneration of the medial compartment of the knee joint in a mouse model.
An animal model of destabilization of the medial meniscus (DMM) was used to induce post-traumatic knee osteoarthritis (OA). PFO was performed to examine the effectiveness of PFO on protection against medial compartment knee OA. Micro-CT was used to observe osteosclerosis development in the subchondral bone, and Safranin O-fast green staining was used to evaluate the progression of articular cartilage destruction. The condylar-plateau angle (CPA) and anatomical femorotibial angle (aFTA) were measured to determine whether knee alignment was changed after PFO.
PFO treatment could decrease osteophyte formation and osteosclerosis development in the subchondral bone, as observed by micro-CT. The value of the ratio of trabecular bone volume to total volume (BV/TV) of DMM+PFO group was lower than that of DMM group. PFO also inhibited the progression of articular cartilage destruction. DMM + PFO group displayed decreased maximal and summed OA scores, as compared with DMM group. Moreover, the change of knee alignment was reduced by PFO, which might be the mechanism of PFO alleviating medial compartment knee OA.
Our results indicated that PFO could alleviate medial compartment knee OA in a mouse model.
本研究旨在建立一种腓骨近端截骨术（PFO）的小鼠模型，并确定 PFO 是否能延缓小鼠模型膝关节内侧间室的退变。
采用内侧半月板不稳定（DMM）动物模型来诱导创伤后膝关节骨关节炎（OA）。进行 PFO 以检验 PFO 对保护膝关节内侧间室 OA 的有效性。采用 micro-CT 观察软骨下骨骨硬化的发展，并用 Safranin O-fast green 染色评估关节软骨破坏的进展。测量髁间隆起角（CPA）和解剖股骨胫骨角（aFTA），以确定 PFO 后膝关节对线是否发生变化。
PFO 治疗可减少软骨下骨骨赘形成和骨硬化的发展，micro-CT 观察到这一点。DMM+PFO 组的骨小梁体积与总体积比值（BV/TV）值低于 DMM 组。PFO 还抑制了关节软骨破坏的进展。与 DMM 组相比，DMM+PFO 组的最大和总和 OA 评分降低。此外，PFO 减少了膝关节对线的变化，这可能是 PFO 缓解膝关节内侧间室 OA 的机制。
我们的结果表明，PFO 可缓解小鼠模型膝关节内侧间室 OA。
