School of Energy & Environment, Southeast University, Nanjing 210096, China.
Taihu Lake Water Environment Engineering Research Center (Wuxi), Southeast University, Wuxi 214061, China.
Int J Environ Res Public Health. 2020 Feb 6;17(3):1025. doi: 10.3390/ijerph17031025.
The judgment and assessment of remediation effect on urban black-odor river still depend on the physical-chemical parameters and lack in ecological safety effects. A set of combined biological toxicity tests were applied to evaluate the ecological effects of one urban black-odor river before and after the remediation. The special growth rate of and mortality rate of were used to assess acute toxicity. The /Reverse Mutation Assay was applied to test the mutagenicity. The tests by growth showed that there was no inhibition before and after remediation by overlying water, in contrast promoted the growth of . The tests by showed slight toxicity on site 3# before remediation and nontoxic after remediation. The mutagenicity of organic extracts from overlying water at all sampling sites were positive before remediation, but were eliminated after remediation except from 3 of 4 sites on TA98 strain. The addition of the liver microsomal S9 induced the positive mutagenicity on site 4# compared to S9 absence. The results clarified the applicable and the importance of the biological toxicity tests on assessing the remediation effect and potential ecological risk of urban black-odor river.
城市黑臭河流的修复效果判断和评估仍然依赖于理化参数,缺乏生态安全效应。采用了一套组合生物毒性试验来评估一条城市黑臭河流在修复前后的生态效应。特殊增长率和死亡率用于评估急性毒性。反向突变试验用于测试致突变性。通过生长试验表明,修复前后的覆盖水没有抑制作用,反而促进了的生长。通过试验表明,修复前 3#点有轻微毒性,修复后无毒。修复前所有采样点的上覆水有机提取物的致突变性均为阳性,但修复后除了 TA98 菌株的 4 个位点中的 3 个外,其他均被消除。与无 S9 相比,肝微粒体 S9 的添加使 4#点的阳性致突变性增加。结果阐明了生物毒性试验在评估城市黑臭河流的修复效果和潜在生态风险方面的适用性和重要性。