Nair M P, Schwartz S A
University of Michigan, Department of Pediatrics, Ann Arbor 48109-2029.
J Allergy Clin Immunol. 1988 Dec;82(6):1089-97. doi: 10.1016/0091-6749(88)90148-0.
Peripheral blood lymphocytes were separated into HNK-1+ and HNK-1- subpopulations and examined for the effects of prednisolone (PRD) on natural killer cell activities in vitro. Preculture of HNK-1+ lymphocytes with PRD (10(-6) to 10(-8) mol/L) for 72 hours resulted in a significant reduction of cytotoxic functions. When peripheral blood lymphocytes were first precultured with PRD and then separated into HNK-1+ and HNK-1- subpopulations, both could suppress the target binding and lytic activities of fresh large granular lymphocytes with the HNK-1+ cells demonstrating greater inhibition than the HNK-1- cells. Moreover, PRD-treated cells demonstrated greater suppression of target binding and cytotoxicity than identical subpopulations cultured without PRD. Culture supernatants of lymphocytes treated with 10(-6) to 10(-9) mol/L concentrations of PRD contain PRD-induced soluble suppressor factor that significantly inhibited the natural killer activity of allogeneic lymphocytes against different targets. PRD-induced soluble suppressor factor was not cytotoxic itself, and suppression was evident at various effector-to-target cell ratios. These studies indicate that in addition to being directly immunosuppressive, corticosteroids may also induce immunoregulatory lymphocytes to secrete a suppressive lymphokine.
将外周血淋巴细胞分离为HNK-1+和HNK-1-亚群，并检测泼尼松龙（PRD）对体外自然杀伤细胞活性的影响。用PRD（10^(-6)至10^(-8)mol/L）预培养HNK-1+淋巴细胞72小时导致细胞毒性功能显著降低。当外周血淋巴细胞先用PRD预培养，然后分离为HNK-1+和HNK-1-亚群时，两者均可抑制新鲜大颗粒淋巴细胞的靶细胞结合和裂解活性，其中HNK-1+细胞的抑制作用比HNK-1-细胞更强。此外，与未用PRD培养的相同亚群相比，经PRD处理的细胞对靶细胞结合和细胞毒性的抑制作用更强。用10^(-6)至10^(-9)mol/L浓度的PRD处理的淋巴细胞培养上清液含有PRD诱导的可溶性抑制因子，该因子可显著抑制同种异体淋巴细胞对不同靶细胞的自然杀伤活性。PRD诱导的可溶性抑制因子本身无细胞毒性，且在各种效应细胞与靶细胞比例下均有明显的抑制作用。这些研究表明，除了具有直接免疫抑制作用外，皮质类固醇还可能诱导免疫调节淋巴细胞分泌抑制性淋巴因子。
