Nair M P, Schwartz S A
Department of Pediatrics, University of Michigan, Ann Arbor 48109.
Clin Immunol Immunopathol. 1988 Oct;49(1):28-40. doi: 10.1016/0090-1229(88)90092-x.
The in vitro effects of Concanavalin A (Con A) and prednisolone (PRD) on the cytotoxic functions of lymphocytes and the generation of lymphokine-activated killer (LAK) cells were investigated. Con A at concentrations ranging from 1 to 40 micrograms/ml did not significantly affect the cytotoxicity of LAK cells when added directly to the effector and target cell mixture in a 4-hr 51Cr release assay. The generation and lytic capacity of LAK cells were significantly affected by Con A in a dose-dependent manner when lectin was added at the initiation of culture. Suppression of LAK cell activity was demonstrable at effector: target (E:T) cell ratios. Lymphocyte cultures incubated with PRD at concentrations ranging from 10(-9) to 10(-4) M showed a decrease in both the numbers of and activity of LAK cells using a variety of target cells. Pretreatment of target cells with either PRD or Con A did not affect their sensitivity to lysis by LAK cells and incubation of lymphocytes with Con A did not induce autoreactive cytotoxic or suppressor cells directed against LAK cell activity. Thus while PRD and Con A can inhibit the generation of LAK cells, they also directly inhibit their specific cytotoxic activity on a per cell basis. These results suggest that like other cytotoxic cells, LAK cells are also under active immunologic control.
研究了伴刀豆球蛋白A（Con A）和泼尼松龙（PRD）对淋巴细胞细胞毒性功能及淋巴因子激活的杀伤（LAK）细胞生成的体外作用。在4小时的51Cr释放试验中，当将浓度为1至40微克/毫升的Con A直接添加到效应细胞和靶细胞混合物中时，它对LAK细胞的细胞毒性没有显著影响。当在培养开始时添加凝集素时，Con A以剂量依赖性方式显著影响LAK细胞的生成和裂解能力。在效应细胞：靶细胞（E:T）比例下可证明LAK细胞活性受到抑制。用浓度范围为10^(-9)至10^(-4) M的PRD孵育淋巴细胞培养物，使用多种靶细胞时，LAK细胞的数量和活性均降低。用PRD或Con A预处理靶细胞不影响它们对LAK细胞裂解的敏感性，并且用Con A孵育淋巴细胞不会诱导针对LAK细胞活性的自身反应性细胞毒性或抑制细胞。因此，虽然PRD和Con A可以抑制LAK细胞的生成，但它们也在单个细胞基础上直接抑制其特异性细胞毒性活性。这些结果表明，与其他细胞毒性细胞一样，LAK细胞也处于活跃的免疫控制之下。
