Giuberti Gianluca, Gallo Antonio
Department for Sustainable Food Process, Università Cattolica del Sacro Cuore, Via Emilia Parmense 84, 29122, Piacenza, Italy.
Department of Animal Science, Food and Nutrition, Università Cattolica del Sacro Cuore, Via Emilia Parmense 84, 29122, Piacenza, Italy.
Heliyon. 2020 Jan 6;6(1):e03145. doi: 10.1016/j.heliyon.2019.e03145. eCollection 2020 Jan.
Four different resistant starch (RS) type 3 (RS3; retrograded starch) and a RS type 2 (RS2; native high amylose maize starch) were digested and fermented by faecal inoculum. Total and individual short chain fatty acid (SCFA) production and associated kinetic parameters were assessed up to 20 h of fermentation. Total SCFA production was different (p < 0.05) among RS-rich ingredients, ranging from 7.43 to 8.72 mmol/g dry starch incubated. Differences (p < 0.05) were recorded for propionate and butyrate productions. Different (p < 0.05) half-time of total SCFA fermentation (T), maximum rate of production (R) and the time of occurrence (T) values were measured among RS-rich ingredients, ranging from 3.3 to 5.6 h, from 1.06 to 1.85 mmol/g dry starch incubated per hour and from 2.6 to 4.9 h, respectively. Similar trends were measured considering the fermentative kinetics of individual SCFA. Present preliminary findings indicated that quantitative and qualitative production of SCFA, and inherent fermentation kinetics, were influenced by the type of RS. These findings are based on an approach, thus requiring trials.
四种不同类型的抗性淀粉3(RS3;回生淀粉)和一种抗性淀粉2(RS2;天然高直链玉米淀粉)被粪便接种物消化和发酵。在长达20小时的发酵过程中评估了总短链脂肪酸(SCFA)和单个短链脂肪酸的产生以及相关动力学参数。富含抗性淀粉的成分之间总SCFA产生量不同(p<0.05),范围为每克干淀粉孵育产生7.43至8.72毫摩尔。丙酸盐和丁酸盐产生量有差异(p<0.05)。富含抗性淀粉的成分之间总SCFA发酵半衰期(T)、最大产生速率(R)和出现时间(T)值不同(p<0.05),分别为3.3至5.6小时、每克干淀粉孵育每小时1.06至1.85毫摩尔和2.6至4.9小时。考虑单个SCFA的发酵动力学也测量到了类似趋势。目前的初步研究结果表明,SCFA的定量和定性产生以及内在发酵动力学受抗性淀粉类型的影响。这些发现基于一种方法,因此需要进一步试验。