Department of Microbiology, Faculty of Science, Mahidol University, Bangkok, Thailand.
Department of Parasitology, Phramongkutklao College of Medicine, Bangkok, Thailand.
Am J Trop Med Hyg. 2020 Apr;102(4):838-843. doi: 10.4269/ajtmh.19-0864.
Human liver fluke infection caused by increases the risk of cholangiocarcinoma (CCA) reported along the Mekong basin including Thailand, Lao People's Democratic Republic (PDR), Cambodia, and Vietnam. The highest incidence of CCA has been reported in northeastern Thailand where liver fluke infection is prevalent. This study aimed to investigate the prevalence of infection in a northeastern-descendent community in rural Sa Kaeo Province, eastern Thailand, using stool examination and molecular technique. The Kato-Katz method was performed to determine eggs per gram (EPG) for infection intensity. Phosphate-buffered saline-ethyl acetate concentration was used to prepare specimens for polymerase chain reaction (PCR) and restriction fragment length polymorphism of the internal transcribed spacer 2 (ITS2) region of the ribosomal RNA. From 1,245 specimens, 105 (8.4%) samples were identified as -like eggs from stool examination, and all positive specimens indicated light infection (< 1,000 EPG). From positive -like egg samples, 55.2% (58/105) were identified as eggs from ITS2-PCR assay for which low infection intensity might result in a negative PCR result (44.8%). Using multiple logistic regression analysis, males were at 3.1 times higher risk of acquiring infection than females. From phylogenetic analysis, in eastern Thailand, nucleotide sequences of were grouped as a monoclade as those isolated from Greater Mekong, Vietnam, Myanmar, and west Siberia. The results revealed that the surveyed community is a low-grade endemic area of infection. Thus, data from this study can be used to improve health-promoting programs and activities to control the infection and its subsequent CCA.
人类华支睾吸虫感染增加了湄公河流域（包括泰国、老挝人民民主共和国、柬埔寨和越南）胆管癌（CCA）的报告风险。CCA 的发病率最高的是在泰国东北部，那里华支睾吸虫感染流行。本研究旨在通过粪便检查和分子技术调查泰国东部沙缴府农村东北部后裔社区的 感染流行率。加藤法用于确定每克粪便（EPG）的感染强度。磷酸盐缓冲生理盐水-乙酸乙酯浓度用于制备聚合酶链反应（PCR）和核糖体 RNA 内转录间隔区 2（ITS2）区域的限制性片段长度多态性的标本。从 1245 个标本中，105 个（8.4%）标本通过粪便检查鉴定为类虫卵，所有阳性标本均显示轻度感染（<1000 EPG）。从阳性类虫卵样本中，55.2%（58/105）被鉴定为 ITS2-PCR 检测的 卵，低感染强度可能导致 PCR 结果呈阴性（44.8%）。使用多因素逻辑回归分析，男性感染 的风险是女性的 3.1 倍。从系统发育分析来看，在泰国东部，核苷酸序列的 被分为一个单系群，与来自大湄公河、越南、缅甸和西西伯利亚的分离株相同。研究结果表明，调查社区是一个低度流行的 感染地区。因此，本研究的数据可用于改善促进健康的方案和活动，以控制感染及其随后的 CCA。
