Department of Surgery, Duke University School of Medicine, Durham, North Carolina.
Department of Surgery, Duke University School of Medicine, Durham, North Carolina.
J Surg Res. 2020 Jun;250:119-124. doi: 10.1016/j.jss.2019.12.042. Epub 2020 Feb 7.
Vascularized composite allograft has emerged as a reconstructive option for patients who have suffered severe tissue loss. Animal models are critical for understanding the unique mechanisms of rejection in vascularized composite allograft. We present a functional mouse model of orthotopic hind limb transplantation using end-to-side anastomoses of the donor aorta and inferior vena cava to the respective recipient vessels. To the best of our knowledge, this approach has not been reported in the scientific literature.
A single surgeon performed all transplants (J.W.). A total of 13 syngeneic and 10 fully mismatched allogeneic transplants were performed without immunosuppression. Skin samples from the grafts were collected at the time of euthanasia.
Five syngeneic mice survived for more than 90 d after transplant. All allografts displayed clinical and histologic signs of acute rejection such as a rash at the time of graft excision. The overall technical success rate of all transplants in this study was 74% (17 of 23).
We demonstrate the feasibility of end-to-side anastomoses of the donor aorta and inferior vena cava with functional recovery of the transplant in a mouse model of orthotopic hind limb transplantation.
对于遭受严重组织损失的患者，血管化复合移植物已成为一种重建选择。动物模型对于理解血管化复合移植物独特的排斥机制至关重要。我们提出了一种使用供体主动脉和下腔静脉端侧吻合术将异体吻合到相应受者血管的原位后肢移植的功能性小鼠模型。据我们所知，这种方法尚未在科学文献中报道。
一位外科医生（J.W.）完成了所有移植手术。共进行了 13 例同基因和 10 例完全不合的同种异体移植，未进行免疫抑制。在安乐死时采集移植物的皮肤样本。
5 只同基因小鼠在移植后超过 90 天存活。所有同种异体移植物均显示出急性排斥的临床和组织学迹象，如移植物切除时出现皮疹。本研究中所有移植的总体技术成功率为 74%（23 例中有 17 例）。
我们证明了在原位后肢移植的小鼠模型中，供体主动脉和下腔静脉的端侧吻合术具有可行性，并实现了移植物的功能恢复。
