Institute of Physics, Czech Academy of Sciences, Na Slovance 2, 182 21, Prague, Czech Republic.
Institute of Macromolecular Chemistry, Czech Academy of Sciences, Heyrovského nám. 2, 162 00, Prague, Czech Republic.
Macromol Biosci. 2020 Mar;20(3):e1900351. doi: 10.1002/mabi.201900351. Epub 2020 Feb 11.
Ultra-low fouling and functionalizable coatings represent emerging surface platforms for various analytical and biomedical applications such as those involving examination of cellular interactions in their native environments. Ultra-low fouling surface platforms as advanced interfaces enabling modulation of behavior of living cells via tuning surface physicochemical properties are presented and studied. The state-of-art ultra-low fouling surface-grafted polymer brushes of zwitterionic poly(carboxybetaine acrylamide), nonionic poly(N-(2-hydroxypropyl)methacrylamide), and random copolymers of carboxybetaine methacrylamide (CBMAA) and HPMAA [p(CBMAA-co-HPMAA)] with tunable molar contents of CBMAA and HPMAA are employed. Using a model Huh7 cell line, a systematic study of surface wettability, swelling, and charge effects on the cell growth, shape, and cytoskeleton distribution is performed. This study reveals that ultra-low fouling interfaces with a high content of zwitterionic moieties (>65 mol%) modulate cell behavior in a distinctly different way compared to coatings with a high content of nonionic HPMAA. These differences are attributed mostly to the surface hydration capabilities. The results demonstrate a high potential of carboxybetaine-rich ultra-low fouling surfaces with high hydration capabilities and minimum background signal interferences to create next-generation bioresponsive interfaces for advanced studies of living objects.
超低污染和功能化涂层代表了新兴的表面平台，可用于各种分析和生物医学应用，例如涉及在天然环境中研究细胞相互作用的应用。本文介绍并研究了超低污染表面平台作为先进的界面，通过调整表面物理化学性质来调节活细胞的行为。使用具有可调摩尔含量的两性离子聚（羧基甜菜碱丙烯酰胺）、非离子聚（N-（2-羟丙基）甲基丙烯酰胺）和羧基甜菜碱甲基丙烯酰胺（CBMAA）与 HPMAA 的无规共聚物（p（CBMAA-co-HPMAA））的最新超低污染表面接枝聚合物刷。通过使用模型 Huh7 细胞系，对表面润湿性、溶胀和电荷对细胞生长、形状和细胞骨架分布的影响进行了系统研究。这项研究表明，与具有高含量非离子 HPMAA 的涂层相比，具有高含量两性离子部分（>65mol%）的超低污染界面以明显不同的方式调节细胞行为。这些差异主要归因于表面水合能力。结果表明，具有高水合能力和最小背景信号干扰的富含羧基甜菜碱的超低污染表面具有很大的潜力，可以为先进的活体研究创建下一代响应生物的界面。
