Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Sciences, Nankai University, Tianjin 300071, People's Republic of China.
Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, College of Life Sciences, Nankai University, Tianjin 300071, People's Republic of China.
Biomacromolecules. 2020 Mar 9;21(3):1243-1253. doi: 10.1021/acs.biomac.9b01707. Epub 2020 Feb 19.
Multifunctional tissue adhesives with excellent adhesion, antibleeding, anti-infection, and wound healing properties are desperately needed in clinical surgery. However, the successful development of multifunctional tissue adhesives that simultaneously possess all these properties remains a challenge. We have prepared a novel chitosan-based hydrogel adhesive by integration of hydrocaffeic acid-modified chitosan (CS-HA) with hydrophobically modified chitosan lactate (hmCS lactate) and characterized its gelation time, mechanical properties, and microstructure. Tissue adhesion properties were evaluated using both pigskin and intestine models. In situ antibleeding efficacy was demonstrated via the rat hemorrhaging liver and full-thickness wound closure models. Good antibacterial activity and anti-infection capability toward and were confirmed using in vitro contact-killing assays and an infected pigskin model. The result of coculturing with 3T3 fibroblast cells indicated that the hydrogels have no significant cytotoxicity. Most importantly, the biocompatible and biodegradable CS-HA/hmCS lactate hydrogel was able to close the wound in a sutureless way and promote wound healing. Our results demonstrate that this hydrogel has great promise for sutureless closure of surgical incisions.
多功能组织粘合剂需要具有优异的黏附性、止血、抗感染和促进伤口愈合的性能，这在临床手术中是非常需要的。然而，成功开发同时具备所有这些性能的多功能组织粘合剂仍然是一个挑战。我们通过将水咖啡酸改性壳聚糖（CS-HA）与疏水性改性壳聚糖乳酸盐（hmCS 乳酸盐）整合，制备了一种新型的壳聚糖基水凝胶粘合剂，并对其凝胶时间、力学性能和微观结构进行了表征。使用猪皮和肠模型评估了组织黏附性能。通过大鼠出血性肝和全层伤口闭合模型证明了原位止血效果。通过体外接触杀伤试验和感染猪皮模型证实了对 和 的良好抗菌活性和抗感染能力。与 3T3 成纤维细胞共培养的结果表明，水凝胶没有明显的细胞毒性。最重要的是，生物相容性和可生物降解的 CS-HA/hmCS 乳酸盐水凝胶能够以无缝合的方式闭合伤口并促进伤口愈合。我们的研究结果表明，这种水凝胶在手术切口的无缝合闭合方面具有很大的应用前景。
