Hunan Key Laboratory of Biomedical Nanomaterials and Devices, College of Life Science and Chemistry, Hunan University of Technology, Zhuzhou, 412007, China.
College of Packaging Materials and Engineering, Hunan University of Technology, Zhuzhou, 412007, China.
Macromol Rapid Commun. 2023 Dec;44(23):e2300325. doi: 10.1002/marc.202300325. Epub 2023 Aug 21.
Biocompatible chitosan-based hydrogels have attracted extensive attention in wound dressing due to their human skin-like tissue characteristics. However, it is a crucial challenge to fabricate chitosan-based hydrogels with versatile properties, including flexibility, stretchability, adhesivity, and antibacterial activity. In this work, a kind of chitosan-based hydrogels with integrated functionalities are facilely prepared by solution polymerization of acrylamide (AAm) and sodium p-styrene sulfonate (SS) in the presence of quaternized carboxymethyl chitosan (QCMCS). Due to the dual cross-linking between QCMCS and P(AAm-co-SS), the optimized QCMCS/P(AAm-co-SS) hydrogel exhibits tough mechanical properties (0.767 MPa tensile stress and 1100% fracture strain) and moderate tissue adhesion (11.4 kPa). Moreover, biological evaluation in vitro illustrated that as-prepared hydrogel possesses satisfactory biocompatibility, hemocompatibility, and excellent antibacterial ability (against S. aureus and E. coli are 98.8% and 97.3%, respectively). Then, the hydrogels are tested in a rat model for bacterial infection incision in vivo, and the results show that they can significantly accelerate epidermal regeneration and wound closure. This is due to their ability to reduce the inflammatory response, promote the formation of collagen deposition and granulation tissue. The proposed chitosan-based antibacterial hydrogels have the potential to be a highly effective wound dressing in clinical wound healing.
具有生物相容性的壳聚糖基水凝胶因其具有类似人体皮肤的组织特性而在伤口敷料中受到广泛关注。然而，制备具有多种性能的壳聚糖基水凝胶，包括柔韧性、拉伸性、粘附性和抗菌活性，是一个关键的挑战。在这项工作中，通过丙烯酰胺（AAm）和对苯乙烯磺酸钠（SS）在季铵化羧甲基壳聚糖（QCMCS）存在下的溶液聚合，简便地制备了具有集成功能的壳聚糖基水凝胶。由于 QCMCS 和 P（AAm-co-SS）之间的双重交联，优化的 QCMCS/P（AAm-co-SS）水凝胶表现出坚韧的机械性能（0.767 MPa 的拉伸应力和 1100%的断裂应变）和适度的组织粘附力（11.4 kPa）。此外，体外生物学评估表明，所制备的水凝胶具有良好的生物相容性、血液相容性和优异的抗菌能力（对金黄色葡萄球菌和大肠杆菌的抗菌率分别为 98.8%和 97.3%）。然后，将水凝胶在大鼠细菌感染切口模型中进行体内测试，结果表明它们可以显著加速表皮再生和伤口闭合。这是由于它们能够减轻炎症反应，促进胶原蛋白沉积和肉芽组织形成。基于壳聚糖的抗菌水凝胶具有成为临床伤口愈合中高效伤口敷料的潜力。
