Department of Cosmetic Science, College of Life and Health Sciences, Hoseo University, Asan, Republic of Korea.
Department of Physiology and Medical Science, School of Medicine, Konkuk University, Chungju, Republic of Korea.
Planta Med. 2020 Mar;86(5):348-355. doi: 10.1055/a-1101-9326. Epub 2020 Feb 11.
is widely reported to be a problematic weed in agricultural areas and is mainly used as an indicator plant for the development of herbicides. However, its bioactivities on skin regeneration and wound healing have not been investigated. In the present study, we investigated the effects of flower absolute on skin wound healing and skin regeneration-related events, that is, proliferation, migration, and collagen biosynthesis, in human fibroblasts and keratinocytes. For this study, we extracted absolute from the flower by solvent extraction and identified the composition of flower absolute using GC/MS analysis. We also tested the effect of flower absolute in CCD986sk fibroblasts and/or HaCaT keratinocytes using the WST assay and 5-bromo-2'-deoxyuridine incorporation assay, Boyden chamber assay, ELISA, sprouting assay, and immunoblotting. GC/MS analysis of flower absolute revealed that it contained 15 compounds. The absolute increased the proliferations of keratinocytes and fibroblasts and the migration of fibroblasts but did not affect cell viabilities. In addition, it enhanced the syntheses of type I and IV collagen in fibroblasts, but not in keratinocytes. The sprouting assay showed increased sprout outgrowth of fibroblasts. In addition, flower absolute induced the phosphorylation of extracellular signal-regulated kinase 1/2 and p38 mitogen-activated protein kinase in fibroblasts. These results indicate that flower absolute may promote skin wound healing/regeneration by inducing the proliferation, migration, and collagen synthesis of fibroblasts, as well as the proliferation of keratinocytes. Therefore, flower absolute may be a potential natural source for cosmetic or pharmaceutical agents that promote skin wound healing/regeneration.
被广泛报道为农业区有问题的杂草，主要用作除草剂开发的指示植物。然而，其在皮肤再生和伤口愈合方面的生物活性尚未得到研究。在本研究中，我们研究了 花绝对物对人成纤维细胞和角质形成细胞的皮肤伤口愈合和皮肤再生相关事件（即增殖、迁移和胶原生物合成）的影响。为此研究，我们通过溶剂萃取从 花中提取绝对物，并通过 GC/MS 分析鉴定 花绝对物的组成。我们还通过 WST 测定法和 5-溴-2'-脱氧尿苷掺入测定法、Boyden 室测定法、ELISA、发芽测定法和免疫印迹法，测试了 花绝对物在 CCD986sk 成纤维细胞和/或 HaCaT 角质形成细胞中的作用。GC/MS 分析表明， 花绝对物含有 15 种化合物。绝对物增加了角质形成细胞和成纤维细胞的增殖以及成纤维细胞的迁移，但不影响细胞活力。此外，它增强了成纤维细胞中 I 型和 IV 型胶原的合成，但对角质形成细胞没有影响。发芽测定表明成纤维细胞的芽出增长增加。此外， 花绝对物诱导成纤维细胞中细胞外信号调节激酶 1/2 和 p38 丝裂原活化蛋白激酶的磷酸化。这些结果表明， 花绝对物可能通过诱导成纤维细胞的增殖、迁移和胶原合成以及角质形成细胞的增殖来促进皮肤伤口愈合/再生。因此， 花绝对物可能是促进皮肤伤口愈合/再生的潜在天然来源，可用于化妆品或药物制剂。
