Division of Cosmetic and Biotechnology, College of Life and Health Sciences, Hoseo University, Asan 31499, Korea.
Department of Physiology and Medical Science, School of Medicine, Konkuk University, Seoul 05029, Korea.
Molecules. 2021 Oct 13;26(20):6172. doi: 10.3390/molecules26206172.
Maxim. (APM) is used in traditional medicine to treat chronic gastritis, rheumatic pain, and duodenal bulbar ulcers. However, it is not known whether APM has epidermis-associated biological activities. Here, we investigated the effects of APM flower absolute (APMFAb) on responses associated with skin wound healing and whitening using epidermal cells. APMFAb was obtained by solvent extraction and its composition was analyzed by GC/MS. Water-soluble tetrazolium salt, 5-bromo-2'-deoxyuridine incorporation, Boyden chamber, sprouting, and enzyme-linked immunosorbent assays and immunoblotting were used to examine the effects of APMFAb on HaCaT keratinocytes and B16BL6 melanoma cells. APMFAb contained five compounds and induced keratinocyte migration, proliferation, and type IV collagen synthesis. APMFAb also induced the phosphorylations of ERK1/2, JNK, p38 mitogen-activated protein kinase, and AKT in keratinocytes. In addition, APMFAb decreased serum-induced B16BL6 cell proliferation and inhibited tyrosinase expression, melanin contents, and microphthalmia-associated transcription factor expression in α-melanocyte-stimulating hormone-stimulated B16BL6 cells. These findings demonstrate that APMFAb has beneficial effects on skin wound healing by promoting the proliferation, migration, and collagen synthesis of keratinocytes and on skin whitening by inhibiting melanin synthesis in melanoma cells. Therefore, we suggest that APMFAb has potential use as a wound healing and skin whitening agent.
芍药花提取物（APMFAb）具有治疗慢性胃炎、风湿痛和十二指肠球部溃疡的传统医学用途。但是，APMFAb 对表皮是否具有生物学活性尚不清楚。在这里，我们研究了 APMFAb 对表皮细胞的皮肤伤口愈合和美白相关反应的影响。通过溶剂萃取获得 APMFAb，并用 GC/MS 分析其组成。使用水溶性四唑盐、5-溴-2'-脱氧尿苷掺入、Boyden 室、发芽和酶联免疫吸附试验和免疫印迹来检测 APMFAb 对 HaCaT 角质形成细胞和 B16BL6 黑素瘤细胞的影响。APMFAb 含有五种化合物，可诱导角质形成细胞迁移、增殖和 IV 型胶原蛋白合成。APMFAb 还诱导角质形成细胞中 ERK1/2、JNK、p38 丝裂原活化蛋白激酶和 AKT 的磷酸化。此外，APMFAb 降低了血清诱导的 B16BL6 细胞增殖，并抑制了 α-促黑素细胞激素刺激的 B16BL6 细胞中酪氨酸酶表达、黑色素含量和小眼畸形相关转录因子表达。这些发现表明，APMFAb 通过促进角质形成细胞的增殖、迁移和胶原蛋白合成，对皮肤伤口愈合具有有益作用，通过抑制黑素细胞合成对皮肤美白具有有益作用。因此，我们认为 APMFAb 具有作为伤口愈合和皮肤美白剂的潜力。
