Institute of Fundamental Medicine and Biology, Kazan Federal University, Kazan 420008, Tartastan, Russia.
Republican Clinical Hospital Ministry of Health Republic of Tatarstan, Kazan 420064, Tatarstan, Russia.
Biomolecules. 2020 Feb 7;10(2):257. doi: 10.3390/biom10020257.
Chronic kidney disease (CKD) is an important public health problem in the world. The aim of our research was to identify novel potential serum biomarkers of renal injury. ELISA assay showed that cytokines and chemokines IL-1β, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12 (p70), IL-13, IL-15, IL-17, Eotaxin, FGFb, G-CSF, GM-CSF, IP-10, MCP-1, MIP-1α, MIP-1β, PDGF-1bb, RANTES, TNF-α and VEGF were significantly higher (R > 0.6, value < 0.05) in the serum of patients with CKD compared to healthy subjects, and they were positively correlated with well-established markers (urea and creatinine). The multiple reaction monitoring (MRM) quantification method revealed that levels of HSP90B2, AAT, IGSF22, CUL5, PKCE, APOA4, APOE, APOA1, CCDC171, CCDC43, VIL1, Antigen KI-67, NKRF, APPBP2, CAPRI and most complement system proteins were increased in serum of CKD patients compared to the healthy group. Among complement system proteins, the C8G subunit was significantly decreased three-fold in patients with CKD. However, only AAT and HSP90B2 were positively correlated with well-established markers and, therefore, could be proposed as potential biomarkers for CKD.
慢性肾脏病（CKD）是全球重要的公共卫生问题。我们的研究目的是确定新的潜在的肾损伤血清生物标志物。ELISA 检测显示，细胞因子和趋化因子 IL-1β、IL-2、IL-4、IL-5、IL-6、IL-7、IL-8、IL-9、IL-10、IL-12（p70）、IL-13、IL-15、IL-17、Eotaxin、FGFb、G-CSF、GM-CSF、IP-10、MCP-1、MIP-1α、MIP-1β、PDGF-1bb、RANTES、TNF-α 和 VEGF 在 CKD 患者血清中显著升高（R > 0.6，P 值 < 0.05），且与已建立的标志物（尿素和肌酐）呈正相关。多重反应监测（MRM）定量方法显示，HSP90B2、AAT、IGSF22、CUL5、PKCE、APOA4、APOE、APOA1、CCDC171、CCDC43、VIL1、抗原 KI-67、NKRF、APPBP2、CAPRI 和大多数补体系统蛋白在 CKD 患者血清中的水平高于健康组。在补体系统蛋白中，CKD 患者的 C8G 亚基显著降低了三倍。然而，只有 AAT 和 HSP90B2 与已建立的标志物呈正相关，因此可以作为 CKD 的潜在生物标志物。
