Functional Genomics and Systems Biology Group, Department of Bioinformatics, Biocenter, Am Hubland, D-97074, Würzburg, Germany.
Department of Microbiology, Biocenter, Am Hubland, D-97074, Würzburg, Germany.
Sci Rep. 2020 Feb 11;10(1):2334. doi: 10.1038/s41598-020-59344-1.
Apart from some model organisms, the interactome of most organisms is largely unidentified. High-throughput experimental techniques to determine protein-protein interactions (PPIs) are resource intensive and highly susceptible to noise. Computational methods of PPI determination can accelerate biological discovery by identifying the most promising interacting pairs of proteins and by assessing the reliability of identified PPIs. Here we present a first in-depth study describing a global view of the ant Camponotus floridanus interactome. Although several ant genomes have been sequenced in the last eight years, studies exploring and investigating PPIs in ants are lacking. Our study attempts to fill this gap and the presented interactome will also serve as a template for determining PPIs in other ants in future. Our C. floridanus interactome covers 51,866 non-redundant PPIs among 6,274 proteins, including 20,544 interactions supported by domain-domain interactions (DDIs), 13,640 interactions supported by DDIs and subcellular localization, and 10,834 high confidence interactions mediated by 3,289 proteins. These interactions involve and cover 30.6% of the entire C. floridanus proteome.
除了一些模式生物外，大多数生物的互作组在很大程度上尚未被识别。确定蛋白质-蛋白质相互作用 (PPI) 的高通量实验技术资源密集且高度容易受到干扰。PPI 确定的计算方法可以通过识别最有前途的相互作用蛋白对，并评估已识别 PPI 的可靠性，从而加速生物学发现。在这里，我们首次进行了深入研究，描述了蚂蚁 Camponotus floridanus 互作组的整体视图。尽管在过去的八年中已经对几个蚂蚁基因组进行了测序，但缺乏对蚂蚁中 PPI 的探索和研究。我们的研究试图填补这一空白，所呈现的互作组也将作为未来确定其他蚂蚁中 PPI 的模板。我们的 C. floridanus 互作组涵盖了 6274 种蛋白质中的 51866 个非冗余 PPI，包括 20544 个由结构域-结构域相互作用 (DDI) 支持的相互作用、13640 个由 DDI 和亚细胞定位支持的相互作用以及 10834 个由 3289 个蛋白质介导的高置信度相互作用。这些相互作用涉及并涵盖了整个 C. floridanus 蛋白质组的 30.6%。
